BCR_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BCR_MOUSE
• UniProt AC: Q6PAJ1
• Protein Name: Breakpoint cluster region protein
• Gene Name: Bcr
• Organism: Mus musculus (Mouse).
• Sequence Length: 1270
• Subcellular Localization: Cell junction, synapse, postsynaptic cell membrane, postsynaptic density .
• Protein Description: GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity (By similarity)..
• Protein Sequence: MVDSVGFAEAWRAQFPDSEPPRMELRSVGDIEQELERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQPPDGAAEPRASAPRPPPAPADGADPAPVEESEARPDGEGSPSKGRSASARRPAAAASADRDDRGPPTSVAALRSNFEKIRKGPAQPGSADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQQSAGQGLGEAPRPHYRGRSSESSCGLDGDYEDAELNPRFLKDNLINANGGNRPPWPPLEYQPYQSIYVGGMMVEGEGKSPLLRSQSTSEQEKRLTWPRRSYSPRSFEDSGGGYTPDCSSNENLTSSEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPTPQCQKRHRQCQVVVSEATIVGVRKTGQIWPSDGDSTFQGEADSSFGTPPGYGCAADQAEEQRRHQDGLPYIDDSPSSSPHLSSKGRGSLASGALDPTKVSELDLEKGLEMRKWVLSGILASEETYLSHLEALLLPMKPLKAAATTSQPVLTSQQIETIFFKVPELYEIHKEFYDGLFPRVQQWSHQQRVGDLFQKLASQLGVYRAFVDNYGVAMETAEKCCQANAQFAEISENLRARSNKDVKDSTTKNSLETLLYKPVDRVTRSTLVLHDLLKHTPSSHPDHSLLQDALRISQNFLSSINEEITPRRQSMTVKKGEHRQLLKDSFMVELVEGARKLRHIFLFTDLLLCTKLKKQSGGKTQQYDCKWYIPLTDLSFQMVDELEALPNIPLVPDEELDALKIKISQIKSDIQREKRANKGSKVMERLRKKLSEQESLLLLMSPSMAFRVHSRNGKSYTFLISSDYERAEWRESIREQQKKCFKSFSLTSVELQMLTNSCVKLQTVHHIPLTINKEDDESPGLYGFLHVIVHSATGFKQSSNLYCTLEVDSFGYFVNKAKTRVYRDTTEPNWNEEFEIELEGSQTLRILCYEKCYNKMKMTKEDGESADKLMGKGQVQLDPQTLQDRDWQRTVIDMNGIEVKLSVKFTSREFSLKRMPSRKQTGVFGVKIAVVTKRERSKVPYIVRQCVEEIERRGMEEVGIYRVSGVATDIQALKAAFDVNNKDVSVMMSEMDVNAIAGTLKLYFRELPEPLFTDEFYPNFAEGIALSDPVAKESCMLNLLLSLPEANLLTFLFLLDHLKRVAEKETVNKMSLHNLATVFGPTLLRPSEKESKLPANPSQPITMTDSWSLEVMSQVQVLLYFLQLEAIPAPDSKRQSILFSTEV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MVDSVGFA -------CCCCCCHH	35.00	-
58	Phosphorylation	QERFRMIYLQTLLAK HHHHHHHHHHHHHHH	5.39	-
93	Phosphorylation	GAAEPRASAPRPPPA CCCCCCCCCCCCCCC	39.83	18846507
113	Phosphorylation	DPAPVEESEARPDGE CCCCCCCCCCCCCCC	24.05	21149613
122	Phosphorylation	ARPDGEGSPSKGRSA CCCCCCCCCCCCCCH	23.00	25521595
124	Phosphorylation	PDGEGSPSKGRSASA CCCCCCCCCCCCHHC	49.81	23684622
128	Phosphorylation	GSPSKGRSASARRPA CCCCCCCCHHCCCCH	34.93	29899451
130	Phosphorylation	PSKGRSASARRPAAA CCCCCCHHCCCCHHH	24.45	29899451
139	Phosphorylation	RRPAAAASADRDDRG CCCHHHHCCCCCCCC	26.44	19854140
170	Phosphorylation	KGPAQPGSADAEKPF CCCCCCCCCCCCCCE	29.65	29899451
178	Phosphorylation	ADAEKPFYVNVEFHH CCCCCCEEEEEEEEC	10.31	20116462
198	Phosphorylation	KVNDKEVSDRISSLG ECCCHHHHHHHHHHH	23.43	-
203	Phosphorylation	EVSDRISSLGSQAMQ HHHHHHHHHHHHHHH	34.09	-
216	Phosphorylation	MQMERKKSQQSAGQG HHHHHHHHHHHHCCC	36.17	25266776
219	Phosphorylation	ERKKSQQSAGQGLGE HHHHHHHHHCCCCCC	27.08	29472430
236	Phosphorylation	RPHYRGRSSESSCGL CCCCCCCCCCCCCCC	41.08	27087446
237	Phosphorylation	PHYRGRSSESSCGLD CCCCCCCCCCCCCCC	39.89	25521595
239	Phosphorylation	YRGRSSESSCGLDGD CCCCCCCCCCCCCCC	32.64	25266776
240	Phosphorylation	RGRSSESSCGLDGDY CCCCCCCCCCCCCCH	14.25	26160508
247	Phosphorylation	SCGLDGDYEDAELNP CCCCCCCHHHCCCCH	22.65	17947660
296	Phosphorylation	MVEGEGKSPLLRSQS EEECCCCCCCCCCCC	31.47	24719451
301	Phosphorylation	GKSPLLRSQSTSEQE CCCCCCCCCCCCHHH	28.58	27087446
303	Phosphorylation	SPLLRSQSTSEQEKR CCCCCCCCCCHHHHC	34.84	27087446
304	Phosphorylation	PLLRSQSTSEQEKRL CCCCCCCCCHHHHCC	28.75	23608596
305	Phosphorylation	LLRSQSTSEQEKRLT CCCCCCCCHHHHCCC	42.39	29550500
312	Phosphorylation	SEQEKRLTWPRRSYS CHHHHCCCCCCCCCC	37.14	-
317	Phosphorylation	RLTWPRRSYSPRSFE CCCCCCCCCCCCCCC	31.10	23737553
318	Phosphorylation	LTWPRRSYSPRSFED CCCCCCCCCCCCCCC	22.38	23737553
319	Phosphorylation	TWPRRSYSPRSFEDS CCCCCCCCCCCCCCC	18.32	23737553
335	Phosphorylation	GGYTPDCSSNENLTS CCCCCCCCCCCCCCC	43.51	25338131
336	Phosphorylation	GYTPDCSSNENLTSS CCCCCCCCCCCCCCC	55.27	22802335
356	Phosphorylation	SGQSSRVSPSPTTYR CCCCCCCCCCCCHHH	20.67	27149854
358	Phosphorylation	QSSRVSPSPTTYRMF CCCCCCCCCCHHHHH	27.63	26060331
360	Phosphorylation	SRVSPSPTTYRMFRD CCCCCCCCHHHHHCC	40.57	28066266
361	Phosphorylation	RVSPSPTTYRMFRDK CCCCCCCHHHHHCCC	16.71	28066266
362	Phosphorylation	VSPSPTTYRMFRDKS CCCCCCHHHHHCCCC	11.54	17934518
369	Phosphorylation	YRMFRDKSRSPSQNS HHHHCCCCCCCCCCC	42.13	29472430
371	Phosphorylation	MFRDKSRSPSQNSQQ HHCCCCCCCCCCCCC	35.67	23684622
373	Phosphorylation	RDKSRSPSQNSQQSF CCCCCCCCCCCCCCC	43.15	23684622
376	Phosphorylation	SRSPSQNSQQSFDSS CCCCCCCCCCCCCCC	23.20	25521595
379	Phosphorylation	PSQNSQQSFDSSSPP CCCCCCCCCCCCCCC	24.31	23684622
382	Phosphorylation	NSQQSFDSSSPPTPQ CCCCCCCCCCCCCHH	30.61	19060867
383	Phosphorylation	SQQSFDSSSPPTPQC CCCCCCCCCCCCHHH	49.02	23684622
384	Phosphorylation	QQSFDSSSPPTPQCQ CCCCCCCCCCCHHHH	38.56	25521595
387	Phosphorylation	FDSSSPPTPQCQKRH CCCCCCCCHHHHHHH	29.40	19060867
418	Phosphorylation	KTGQIWPSDGDSTFQ ECCCCCCCCCCCCCC	38.95	26643407
422	Phosphorylation	IWPSDGDSTFQGEAD CCCCCCCCCCCCCCC	36.33	26643407
423	Phosphorylation	WPSDGDSTFQGEADS CCCCCCCCCCCCCCC	25.39	26643407
430	Phosphorylation	TFQGEADSSFGTPPG CCCCCCCCCCCCCCC	34.32	26643407
431	Phosphorylation	FQGEADSSFGTPPGY CCCCCCCCCCCCCCC	28.39	26643407
434	Phosphorylation	EADSSFGTPPGYGCA CCCCCCCCCCCCCCC	25.05	26643407
438	Phosphorylation	SFGTPPGYGCAADQA CCCCCCCCCCCHHHH	18.14	26643407
457	Phosphorylation	RHQDGLPYIDDSPSS HHCCCCCCCCCCCCC	23.25	25619855
461	Phosphorylation	GLPYIDDSPSSSPHL CCCCCCCCCCCCCCC	23.96	27087446
463	Phosphorylation	PYIDDSPSSSPHLSS CCCCCCCCCCCCCCC	47.61	27087446
464	Phosphorylation	YIDDSPSSSPHLSSK CCCCCCCCCCCCCCC	51.80	27087446
465	Phosphorylation	IDDSPSSSPHLSSKG CCCCCCCCCCCCCCC	22.22	27087446
469	Phosphorylation	PSSSPHLSSKGRGSL CCCCCCCCCCCCCCC	27.28	25619855
470	Phosphorylation	SSSPHLSSKGRGSLA CCCCCCCCCCCCCCC	45.55	25619855
473	Methylation	PHLSSKGRGSLASGA CCCCCCCCCCCCCCC	34.95	24129315
475	Phosphorylation	LSSKGRGSLASGALD CCCCCCCCCCCCCCC	21.28	26824392
478	Phosphorylation	KGRGSLASGALDPTK CCCCCCCCCCCCCCC	29.86	26160508
484	Phosphorylation	ASGALDPTKVSELDL CCCCCCCCCCCCCCH	43.68	26804993
487	Phosphorylation	ALDPTKVSELDLEKG CCCCCCCCCCCHHHC	33.72	-
553	Phosphorylation	FFKVPELYEIHKEFY EEECHHHHHHHHHHH	15.92	-
560	Phosphorylation	YEIHKEFYDGLFPRV HHHHHHHHHCCHHHH	15.36	-
590	Phosphorylation	LASQLGVYRAFVDNY HHHHHCHHHHHHHHH	8.40	-
637	Phosphorylation	KDSTTKNSLETLLYK CCCCCHHHHHHHHCC	29.19	26370283
640	Phosphorylation	TTKNSLETLLYKPVD CCHHHHHHHHCCCCC	27.62	22705319
643	Phosphorylation	NSLETLLYKPVDRVT HHHHHHHCCCCCHHH	19.16	17164333
692	Phosphorylation	SSINEEITPRRQSMT HHCCCCCCCCCCCCC	17.64	-
697	Phosphorylation	EITPRRQSMTVKKGE CCCCCCCCCCCCCCC	18.01	29899451
699	Phosphorylation	TPRRQSMTVKKGEHR CCCCCCCCCCCCCHH	34.13	28066266
755	Phosphorylation	QQYDCKWYIPLTDLS EEEEEEEEEECCCCE	4.34	-
843	Phosphorylation	HSRNGKSYTFLISSD ECCCCCEEEEEECCC	12.66	-
851	Phosphorylation	TFLISSDYERAEWRE EEEECCCHHHHHHHH	14.84	-
872	Phosphorylation	KKCFKSFSLTSVELQ HHHHHHCCCCHHHHH	37.52	22802335
882	Phosphorylation	SVELQMLTNSCVKLQ HHHHHHHHCCCCEEE	21.53	22802335
984	Acetylation	EKCYNKMKMTKEDGE HHHHHCCCCCCCCCH	45.06	7718175
1034	Phosphorylation	KLSVKFTSREFSLKR EEEEEEECCCEEEEC	32.71	22324799
1038	Phosphorylation	KFTSREFSLKRMPSR EEECCCEEEECCCCC	28.38	29514104
1048	Phosphorylation	RMPSRKQTGVFGVKI CCCCCCCCCCCCEEE	38.05	25338131
1112	Phosphorylation	DVNNKDVSVMMSEMD CCCCCCCEEEEEHHH	17.90	26160508
1116	Phosphorylation	KDVSVMMSEMDVNAI CCCEEEEEHHHHHHH	16.21	26160508
1259	Phosphorylation	EAIPAPDSKRQSILF HCCCCCCCCCCEEEE	28.78	-
1263	Phosphorylation	APDSKRQSILFSTEV CCCCCCCEEEEEECC	25.87	25521595
1267	Phosphorylation	KRQSILFSTEV---- CCCEEEEEECC----	21.88	23984901
1268	Phosphorylation	RQSILFSTEV----- CCEEEEEECC-----	33.17	25777480

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
178	Y	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
178	Y	Phosphorylation	Kinase	HCK	P08103	Uniprot
247	Y	Phosphorylation	Kinase	FES	P16879	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of BCR_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BCR_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RAC1_MOUSE	Rac1	physical	17116687

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-461, AND MASSSPECTROMETRY.
PubMed: 19367708

"Quantitative time-resolved phosphoproteomic analysis of mast cellsignaling.";
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y.,Kawakami T., Salomon A.R.;
J. Immunol. 179:5864-5876(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-237 AND TYR-247, ANDMASS SPECTROMETRY.
PubMed: 17947660