BACD2_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BACD2_RAT
• UniProt AC: Q7TNY1
• Protein Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• Gene Name: Tnfaip1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 316
• Subcellular Localization: Cytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes..
• Protein Description: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis (By similarity). May enhance the PCNA-dependent DNA polymerase delta activity..
• Protein Sequence: MSGDTCLCPASGAKPKISGFKGGGLGNKYVQLNVGGSLHYTTVRALTRHDTMLKAMFSGRMEVLTDKEGWILIDRCGKHFGTILNYLRDDTVTLPQSRQEIQELMAEAKYYLIQGLVSLCQAALQDKKDSYQPVCNIPIITSLREEDRLIESSTKPVVKLLYNRSNNKYSYTSNSDDHLLKNIELFDKLSLRFNGRVLFIKDVIGDEICCWSFYGQGRKLAEVCCTSIVYATEKKQTKVEFPEARIYEETLNVLLYETPRVPDNSLLEATSRSRSQASPSEDEDTFELRDRVRRIHVKRYSTYDDRQLGHQSAHRD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
141	Phosphorylation	VCNIPIITSLREEDR CCCCCEEECCCHHHH	23.56	-
142	Phosphorylation	CNIPIITSLREEDRL CCCCEEECCCHHHHH	18.28	-
275	Phosphorylation	EATSRSRSQASPSED HHHHCCCCCCCCCCC	31.20	25575281
278	Phosphorylation	SRSRSQASPSEDEDT HCCCCCCCCCCCCCH	22.71	22108457
280	Phosphorylation	SRSQASPSEDEDTFE CCCCCCCCCCCCHHH	56.00	28689409
285	Phosphorylation	SPSEDEDTFELRDRV CCCCCCCHHHHHHHH	19.57	25575281

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
280	S	Phosphorylation	Kinase	CK2	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
280	S	Phosphorylation	Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BACD2_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PCNA_MOUSE	Pcna	physical	15726626

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.