dbPTM

AT1A3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AT1A3_MOUSE
UniProt AC	Q6PIC6
Protein Name	Sodium/potassium-transporting ATPase subunit alpha-3
Gene Name	Atp1a3
Organism	Mus musculus (Mouse).
Sequence Length	1013
Subcellular Localization	Cell membrane Multi-pass membrane protein.
Protein Description	This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity)..
Protein Sequence	MGDKKDDKSSPKKSKAKERRDLDDLKKEVAMTEHKMSVEEVCRKYNTDCVQGLTHSKAQEILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAILCFLAYGIQAGTEDDPSGDNLYLGIVLAAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIREGEKMQVNAEEVVVGDLVEIKGGDRVPADLRIISAHGCKVDNSSLTGESEPQTRSPDCTHDNPLETRNITFFSTNCVEGTARGVVVATGDRTVMGRIATLASGLEVGKTPIAIEIEHFIQLITGVAVFLGVSFFILSLILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGTSFDKSSHTWVALSHIAGLCNRAVFKGGQDNIPVLKRDVAGDASESALLKCIELSSGSVKLMRERNKKVAEIPFNSTNKYQLSIHETEDPNDNRYLLVMKGAPERILDRCATILLQGKEQPLDEEMKEAFQNAYLELGGLGERVLGFCHYYLPEEQFPKGFAFDCDDVNFTTDNLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVIHGTDLKDFTSEQIDEILQNHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIMANIPLPLGTITILCIDLGTDMVPAISLAYEAAESDIMKRQPRNPRTDKLVNERLISMAYGQIGMIQALGGFFSYFVILAENGFLPGNLVGIRLNWDDRTVNDLEDSYGQQWTYEQRKVVEFTCHTAFFVSIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMDVALRMYPLKPSWWFCAFPYSFLIFVYDEIRKLILRRNPGGWVEKETYY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	GDKKDDKSSPKKSKA CCCCCCCCCCCHHHH	59.89	20415495
10	Phosphorylation	DKKDDKSSPKKSKAK CCCCCCCCCCHHHHH	46.62	20415495
26	Ubiquitination	RRDLDDLKKEVAMTE HCCHHHHHHHHHHHH	54.61	22790023
27	Ubiquitination	RDLDDLKKEVAMTEH CCHHHHHHHHHHHHH	65.79	22790023
32	Phosphorylation	LKKEVAMTEHKMSVE HHHHHHHHHHCCCHH	25.79	29899451
35	Ubiquitination	EVAMTEHKMSVEEVC HHHHHHHCCCHHHHH	27.00	22790023
37	Phosphorylation	AMTEHKMSVEEVCRK HHHHHCCCHHHHHHH	31.05	19060867
44	Ubiquitination	SVEEVCRKYNTDCVQ CHHHHHHHHCCCCCC	37.41	22790023
45	Nitration	VEEVCRKYNTDCVQG HHHHHHHHCCCCCCC	12.16	-
45	Phosphorylation	VEEVCRKYNTDCVQG HHHHHHHHCCCCCCC	12.16	24759943
47	Phosphorylation	EVCRKYNTDCVQGLT HHHHHHCCCCCCCCC	27.90	25367039
49	S-nitrosylation	CRKYNTDCVQGLTHS HHHHCCCCCCCCCHH	2.01	24895380
54	Phosphorylation	TDCVQGLTHSKAQEI CCCCCCCCHHHHHHH	30.81	25367039
56	Phosphorylation	CVQGLTHSKAQEILA CCCCCCHHHHHHHHH	25.17	25521595
57	Ubiquitination	VQGLTHSKAQEILAR CCCCCHHHHHHHHHC	46.62	22790023
71	Phosphorylation	RDGPNALTPPPTTPE CCCCCCCCCCCCCHH	31.19	22817900
75	Phosphorylation	NALTPPPTTPEWVKF CCCCCCCCCHHHHHH	61.84	20415495
76	Phosphorylation	ALTPPPTTPEWVKFC CCCCCCCCHHHHHHH	25.61	25521595
83	S-palmitoylation	TPEWVKFCRQLFGGF CHHHHHHHHHHHCHH	1.94	28680068
146	Ubiquitination	YQEAKSSKIMESFKN HHHHHHHHHHHHHHH	53.84	-
150	Phosphorylation	KSSKIMESFKNMVPQ HHHHHHHHHHHCCCC	24.92	22324799
152	Ubiquitination	SKIMESFKNMVPQQA HHHHHHHHHCCCCCE	54.11	22790023
167	Ubiquitination	LVIREGEKMQVNAEE EEEECCCEEECCCEE	45.55	22790023
184	Ubiquitination	VGDLVEIKGGDRVPA EECEEEECCCCCCCC	43.25	22790023
197	Phosphorylation	PADLRIISAHGCKVD CCCEEEEEECCCEEC	16.31	22324799
201	S-nitrosylation	RIISAHGCKVDNSSL EEEEECCCEECCCCC	2.52	24895380
202	Ubiquitination	IISAHGCKVDNSSLT EEEECCCEECCCCCC	58.16	22790023
206	Phosphorylation	HGCKVDNSSLTGESE CCCEECCCCCCCCCC	23.43	25521595
207	Phosphorylation	GCKVDNSSLTGESEP CCEECCCCCCCCCCC	35.88	25521595
209	Phosphorylation	KVDNSSLTGESEPQT EECCCCCCCCCCCCC	40.63	27742792
212	Phosphorylation	NSSLTGESEPQTRSP CCCCCCCCCCCCCCC	56.79	25521595
216	Phosphorylation	TGESEPQTRSPDCTH CCCCCCCCCCCCCCC	43.78	25521595
218	Phosphorylation	ESEPQTRSPDCTHDN CCCCCCCCCCCCCCC	28.60	25521595
221	S-nitrosylation	PQTRSPDCTHDNPLE CCCCCCCCCCCCCCC	4.06	24895380
222	Phosphorylation	QTRSPDCTHDNPLET CCCCCCCCCCCCCCC	39.38	29899451
237	Phosphorylation	RNITFFSTNCVEGTA CCEEEEECCCCCCCC	27.72	-
239	S-nitrosylation	ITFFSTNCVEGTARG EEEEECCCCCCCCCE	2.74	24895380
255	Phosphorylation	VVATGDRTVMGRIAT EEECCCCCHHHHHHH	21.42	-
262	Phosphorylation	TVMGRIATLASGLEV CHHHHHHHHHHCCCC	22.08	20415495
265	Phosphorylation	GRIATLASGLEVGKT HHHHHHHHCCCCCCC	47.20	25521595
349	Ubiquitination	ARKNCLVKNLEAVET HHCCCEECCHHHHHH	42.75	-
356	Phosphorylation	KNLEAVETLGSTSTI CCHHHHHHHCCCCCC	29.80	23140645
359	Phosphorylation	EAVETLGSTSTICSD HHHHHHCCCCCCCCC	23.46	25521595
360	Phosphorylation	AVETLGSTSTICSDK HHHHHCCCCCCCCCC	28.26	24925903
361	Phosphorylation	VETLGSTSTICSDKT HHHHCCCCCCCCCCC	19.92	23140645
362	Phosphorylation	ETLGSTSTICSDKTG HHHCCCCCCCCCCCC	26.54	24925903
364	S-nitrosylation	LGSTSTICSDKTGTL HCCCCCCCCCCCCCC	4.33	24895380
365	Phosphorylation	GSTSTICSDKTGTLT CCCCCCCCCCCCCCC	37.99	25521595
367	Acetylation	TSTICSDKTGTLTQN CCCCCCCCCCCCCCC	32.34	6569677
367	Ubiquitination	TSTICSDKTGTLTQN CCCCCCCCCCCCCCC	32.34	-
368	Phosphorylation	STICSDKTGTLTQNR CCCCCCCCCCCCCCC	40.15	25521595
370	Phosphorylation	ICSDKTGTLTQNRMT CCCCCCCCCCCCCEE	31.68	22324799
372	Phosphorylation	SDKTGTLTQNRMTVA CCCCCCCCCCCEEEE	24.52	24925903
399	Phosphorylation	TTEDQSGTSFDKSSH CCCCCCCCCCCCCHH	31.31	25521595
400	Phosphorylation	TEDQSGTSFDKSSHT CCCCCCCCCCCCHHH	34.67	29899451
404	Phosphorylation	SGTSFDKSSHTWVAL CCCCCCCCHHHHHHH	29.24	29899451
405	Phosphorylation	GTSFDKSSHTWVALS CCCCCCCHHHHHHHH	30.58	29899451
418	S-nitrosylation	LSHIAGLCNRAVFKG HHHHHHHHCCHHHCC	2.95	24895380
424	Ubiquitination	LCNRAVFKGGQDNIP HHCCHHHCCCCCCCC	56.38	22790023
434	Acetylation	QDNIPVLKRDVAGDA CCCCCEECCCCCCCC	46.95	130057
434	Ubiquitination	QDNIPVLKRDVAGDA CCCCCEECCCCCCCC	46.95	22790023
442	Phosphorylation	RDVAGDASESALLKC CCCCCCCCHHHHHHH	36.50	25521595
444	Phosphorylation	VAGDASESALLKCIE CCCCCCHHHHHHHHH	23.01	22817900
448	Ubiquitination	ASESALLKCIELSSG CCHHHHHHHHHHCCC	33.73	22790023
449	S-nitrosylation	SESALLKCIELSSGS CHHHHHHHHHHCCCC	2.70	24895380
453	Phosphorylation	LLKCIELSSGSVKLM HHHHHHHCCCCHHHH	21.62	20415495
454	Phosphorylation	LKCIELSSGSVKLMR HHHHHHCCCCHHHHH	47.95	22324799
456	Phosphorylation	CIELSSGSVKLMRER HHHHCCCCHHHHHHH	20.30	22324799
458	Acetylation	ELSSGSVKLMRERNK HHCCCCHHHHHHHCC	37.96	6568705
458	Ubiquitination	ELSSGSVKLMRERNK HHCCCCHHHHHHHCC	37.96	22790023
466	Ubiquitination	LMRERNKKVAEIPFN HHHHHCCCEEECCCC	50.67	22790023
474	Phosphorylation	VAEIPFNSTNKYQLS EEECCCCCCCCEEEE	33.46	25521595
475	Phosphorylation	AEIPFNSTNKYQLSI EECCCCCCCCEEEEE	36.51	25521595
477	Ubiquitination	IPFNSTNKYQLSIHE CCCCCCCCEEEEEEE	34.17	22790023
493	Phosphorylation	EDPNDNRYLLVMKGA CCCCCCEEEEEECCC	15.61	18034455
498	Ubiquitination	NRYLLVMKGAPERIL CEEEEEECCCHHHHH	44.43	22790023
508	S-nitrosylation	PERILDRCATILLQG HHHHHHHHHHHHHCC	3.80	24895380
516	Ubiquitination	ATILLQGKEQPLDEE HHHHHCCCCCCCCHH	39.97	22790023
525	Ubiquitination	QPLDEEMKEAFQNAY CCCCHHHHHHHHHHH	49.52	-
532	Nitration	KEAFQNAYLELGGLG HHHHHHHHHHHCCHH	14.30	-
532	Phosphorylation	KEAFQNAYLELGGLG HHHHHHHHHHHCCHH	14.30	20415495
546	S-nitrosylation	GERVLGFCHYYLPEE HHHHHHHHCEECCHH	1.59	22588120
548	Phosphorylation	RVLGFCHYYLPEEQF HHHHHHCEECCHHHC	14.10	19060867
549	Phosphorylation	VLGFCHYYLPEEQFP HHHHHCEECCHHHCC	7.66	19060867
580	Phosphorylation	LCFVGLMSMIDPPRA CEEEHHHHCCCCCHH	20.03	-
595	Ubiquitination	AVPDAVGKCRSAGIK CCCCHHHHHHHCCCE	21.63	-
598	Phosphorylation	DAVGKCRSAGIKVIM CHHHHHHHCCCEEEE	40.07	29899451
607	O-linked_Glycosylation	GIKVIMVTGDHPITA CCEEEEEECCCCCCH	21.36	51462369
607	Phosphorylation	GIKVIMVTGDHPITA CCEEEEEECCCCCCH	21.36	28638064
613	Phosphorylation	VTGDHPITAKAIAKG EECCCCCCHHHHHHC	26.93	-
615	Ubiquitination	GDHPITAKAIAKGVG CCCCCCHHHHHHCCE	31.51	-
619	Ubiquitination	ITAKAIAKGVGIISE CCHHHHHHCCEEEEC	48.27	-
625	Phosphorylation	AKGVGIISEGNETVE HHCCEEEECCCCCHH	37.21	22324799
630	Phosphorylation	IISEGNETVEDIAAR EEECCCCCHHHHHHH	32.94	25521595
643	O-linked_Glycosylation	ARLNIPVSQVNPRDA HHCCCCHHHCCHHHC	24.05	179351
643	Phosphorylation	ARLNIPVSQVNPRDA HHCCCCHHHCCHHHC	24.05	27180971
651	Ubiquitination	QVNPRDAKACVIHGT HCCHHHCCEEEEECC	46.75	22790023
653	S-nitrosylation	NPRDAKACVIHGTDL CHHHCCEEEEECCCH	2.66	24895380
658	Phosphorylation	KACVIHGTDLKDFTS CEEEEECCCHHHCCH	24.81	25521595
661	Ubiquitination	VIHGTDLKDFTSEQI EEECCCHHHCCHHHH	54.31	-
665	Phosphorylation	TDLKDFTSEQIDEIL CCHHHCCHHHHHHHH	27.74	-
676	Phosphorylation	DEILQNHTEIVFART HHHHHHCCEEEEEEC	34.65	29899451
688	Ubiquitination	ARTSPQQKLIIVEGC EECCCCCEEEEEECC	36.08	-
695	S-nitrosylation	KLIIVEGCQRQGAIV EEEEEECCCCCCCEE	1.58	24895380
705	Phosphorylation	QGAIVAVTGDGVNDS CCCEEEEECCCCCCC	21.59	24925903
712	Phosphorylation	TGDGVNDSPALKKAD ECCCCCCCHHHHHCC	13.58	25521595
716	Acetylation	VNDSPALKKADIGVA CCCCHHHHHCCCEEE	48.28	130061
716	Ubiquitination	VNDSPALKKADIGVA CCCCHHHHHCCCEEE	48.28	-
717	Ubiquitination	NDSPALKKADIGVAM CCCHHHHHCCCEEEC	52.74	22790023
763	Ubiquitination	RLIFDNLKKSIAYTL EEEECCHHHHHHHHH	51.21	-
819	Phosphorylation	LAYEAAESDIMKRQP HHHHHHHHHHHHCCC	27.92	-
831	Phosphorylation	RQPRNPRTDKLVNER CCCCCCCCHHHHHHH	38.45	-
833	Ubiquitination	PRNPRTDKLVNERLI CCCCCCHHHHHHHHH	54.44	22790023
933	Phosphorylation	ICKTRRNSVFQQGMK EECCCCCHHHHHHCC	23.61	19060867
940	Ubiquitination	SVFQQGMKNKILIFG HHHHHHCCCCEEEEE	63.01	-
1009	Ubiquitination	NPGGWVEKETYY--- CCCCCCEEECCC---	45.82	22790023
1012	Phosphorylation	GWVEKETYY------ CCCEEECCC------	14.31	18034455
1013	Phosphorylation	WVEKETYY------- CCEEECCC-------	21.35	18034455

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
933	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AT1A3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AT1A3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of AT1A3_MOUSE !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AT1A3_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative analysis of both protein expression and serine /threonine post-translational modifications through stable isotopelabeling with dithiothreitol."; Vosseller K., Hansen K.C., Chalkley R.J., Trinidad J.C., Wells L.,Hart G.W., Burlingame A.L.; Proteomics 5:388-398(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-265, AND MASSSPECTROMETRY.	15648052 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-368, AND MASSSPECTROMETRY.	17242355 [Abstract]
"Large-scale identification and evolution indexing of tyrosinephosphorylation sites from murine brain."; Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; J. Proteome Res. 7:311-318(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-493; TYR-548 ANDTYR-549, AND MASS SPECTROMETRY.	18034455 [Abstract]