ASSY_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ASSY_MOUSE
• UniProt AC: P16460
• Protein Name: Argininosuccinate synthase {ECO:0000305}
• Gene Name: Ass1 {ECO:0000312|MGI:MGI:88090}
• Organism: Mus musculus (Mouse).
• Sequence Length: 412
• Subcellular Localization: Cytoplasm, cytosol .
• Protein Description: One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues..
• Protein Sequence: MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSKEFVEEFIWPAVQSSALYEDRYLLGTSLARPCIARRQVEIAQREGAKYVSHGATGKGNDQVRFELTCYSLAPQIKVIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKSPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNSPDVLEIEFKKGVPVKVTNIKDGTTRTTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIQKSQERVEGKVQVSVFKGQVYILGRESPLSLYNEELVSMNVQGDYEPIDATGFININSLRLKEYHRLQSKVTAK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
29	Phosphorylation	VWLKEQGYDVIAYLA HHHHHCCCCHHHHHH	13.51	25195567
41	Acetylation	YLANIGQKEDFEEAR HHHCCCCCHHHHHHH	54.78	22733758
41	Malonylation	YLANIGQKEDFEEAR HHHCCCCCHHHHHHH	54.78	26073543
41	Ubiquitination	YLANIGQKEDFEEAR HHHCCCCCHHHHHHH	54.78	-
53	Acetylation	EARKKALKLGAKKVF HHHHHHHHHCCCEEE	50.13	23864654
53	Malonylation	EARKKALKLGAKKVF HHHHHHHHHCCCEEE	50.13	26320211
58	Ubiquitination	ALKLGAKKVFIEDVS HHHHCCCEEEEEHHC	41.67	-
58	Acetylation	ALKLGAKKVFIEDVS HHHHCCCEEEEEHHC	41.67	23576753
58	Glutarylation	ALKLGAKKVFIEDVS HHHHCCCEEEEEHHC	41.67	24703693
58	Malonylation	ALKLGAKKVFIEDVS HHHHCCCEEEEEHHC	41.67	26073543
79	Phosphorylation	FIWPAVQSSALYEDR HHHHHHHCCCCCCCH	15.81	23984901
80	Phosphorylation	IWPAVQSSALYEDRY HHHHHHCCCCCCCHH	12.76	23984901
83	Phosphorylation	AVQSSALYEDRYLLG HHHCCCCCCCHHHHC	18.59	23984901
87	Phosphorylation	SALYEDRYLLGTSLA CCCCCCHHHHCCCCH	20.71	25195567
91	Phosphorylation	EDRYLLGTSLARPCI CCHHHHCCCCHHHHH	22.12	20469934
92	Phosphorylation	DRYLLGTSLARPCIA CHHHHCCCCHHHHHH	20.45	23140645
97	S-palmitoylation	GTSLARPCIARRQVE CCCCHHHHHHHHHHH	2.94	28526873
112	Ubiquitination	IAQREGAKYVSHGAT HHHHHCCEEEECCCC	57.97	-
112	Acetylation	IAQREGAKYVSHGAT HHHHHCCEEEECCCC	57.97	23576753
112	Glutarylation	IAQREGAKYVSHGAT HHHHHCCEEEECCCC	57.97	24703693
112	Malonylation	IAQREGAKYVSHGAT HHHHHCCEEEECCCC	57.97	26073543
113	Phosphorylation	AQREGAKYVSHGATG HHHHCCEEEECCCCC	13.47	29899451
115	Phosphorylation	REGAKYVSHGATGKG HHCCEEEECCCCCCC	17.08	22324799
119	Phosphorylation	KYVSHGATGKGNDQV EEEECCCCCCCCCCE	43.98	22324799
121	Glutarylation	VSHGATGKGNDQVRF EECCCCCCCCCCEEE	50.62	24703693
121	Malonylation	VSHGATGKGNDQVRF EECCCCCCCCCCEEE	50.62	26073543
121	Ubiquitination	VSHGATGKGNDQVRF EECCCCCCCCCCEEE	50.62	-
121	Acetylation	VSHGATGKGNDQVRF EECCCCCCCCCCEEE	50.62	23864654
131	Phosphorylation	DQVRFELTCYSLAPQ CCEEEEEEEEECCCC	11.38	25195567
132	S-palmitoylation	QVRFELTCYSLAPQI CEEEEEEEEECCCCE	3.21	28526873
133	Phosphorylation	VRFELTCYSLAPQIK EEEEEEEEECCCCEE	11.07	29472430
134	Phosphorylation	RFELTCYSLAPQIKV EEEEEEEECCCCEEE	21.87	17242355
140	Ubiquitination	YSLAPQIKVIAPWRM EECCCCEEEEECCCC	23.49	-
140	Malonylation	YSLAPQIKVIAPWRM EECCCCEEEEECCCC	23.49	26073543
151	Phosphorylation	PWRMPEFYNRFKGRN CCCCHHHHHHCCCHH	12.08	17242355
155	Ubiquitination	PEFYNRFKGRNDLME HHHHHHCCCHHHHHH	54.44	22790023
161	Sulfoxidation	FKGRNDLMEYAKQHG CCCHHHHHHHHHHHC	4.06	21406390
163	Phosphorylation	GRNDLMEYAKQHGIP CHHHHHHHHHHHCCC	12.72	28576409
165	Acetylation	NDLMEYAKQHGIPIP HHHHHHHHHHCCCCC	41.76	23864654
165	Ubiquitination	NDLMEYAKQHGIPIP HHHHHHHHHHCCCCC	41.76	-
165	Malonylation	NDLMEYAKQHGIPIP HHHHHHHHHHCCCCC	41.76	26320211
174	Phosphorylation	HGIPIPVTPKSPWSM HCCCCCCCCCCCCCC	21.46	22324799
176	Ubiquitination	IPIPVTPKSPWSMDE CCCCCCCCCCCCCCC	60.84	-
176	Acetylation	IPIPVTPKSPWSMDE CCCCCCCCCCCCCCC	60.84	-
176	Malonylation	IPIPVTPKSPWSMDE CCCCCCCCCCCCCCC	60.84	26320211
177	Phosphorylation	PIPVTPKSPWSMDEN CCCCCCCCCCCCCCC	32.57	21082442
180	Phosphorylation	VTPKSPWSMDENLMH CCCCCCCCCCCCCCC	21.53	26643407
189	Phosphorylation	DENLMHISYEAGILE CCCCCCEEHHHCCCC	11.40	23140645
190	Phosphorylation	ENLMHISYEAGILEN CCCCCEEHHHCCCCC	14.81	23140645
199	Ubiquitination	AGILENPKNQAPPGL HCCCCCCCCCCCCCC	73.59	-
199	Malonylation	AGILENPKNQAPPGL HCCCCCCCCCCCCCC	73.59	26320211
207	Phosphorylation	NQAPPGLYTKTQDPA CCCCCCCCCCCCCCC	16.47	25195567
209	Ubiquitination	APPGLYTKTQDPAKA CCCCCCCCCCCCCCC	30.54	-
209	Malonylation	APPGLYTKTQDPAKA CCCCCCCCCCCCCCC	30.54	26320211
210	Phosphorylation	PPGLYTKTQDPAKAP CCCCCCCCCCCCCCC	30.33	21183079
215	Malonylation	TKTQDPAKAPNSPDV CCCCCCCCCCCCCCE	70.53	26320211
215	Ubiquitination	TKTQDPAKAPNSPDV CCCCCCCCCCCCCCE	70.53	-
215	Acetylation	TKTQDPAKAPNSPDV CCCCCCCCCCCCCCE	70.53	23864654
219	Phosphorylation	DPAKAPNSPDVLEIE CCCCCCCCCCEEEEE	22.78	25521595
228	Acetylation	DVLEIEFKKGVPVKV CEEEEEEECCCCEEE	35.51	23954790
228	Ubiquitination	DVLEIEFKKGVPVKV CEEEEEEECCCCEEE	35.51	-
228	Malonylation	DVLEIEFKKGVPVKV CEEEEEEECCCCEEE	35.51	26320211
229	Malonylation	VLEIEFKKGVPVKVT EEEEEEECCCCEEEE	71.15	25418362
229	Acetylation	VLEIEFKKGVPVKVT EEEEEEECCCCEEEE	71.15	156747
234	Malonylation	FKKGVPVKVTNIKDG EECCCCEEEEECCCC	36.95	26320211
234	Ubiquitination	FKKGVPVKVTNIKDG EECCCCEEEEECCCC	36.95	-
239	Malonylation	PVKVTNIKDGTTRTT CEEEEECCCCCCEEE	52.77	26073543
239	Acetylation	PVKVTNIKDGTTRTT CEEEEECCCCCCEEE	52.77	22733758
239	Ubiquitination	PVKVTNIKDGTTRTT CEEEEECCCCCCEEE	52.77	-
239	Glutarylation	PVKVTNIKDGTTRTT CEEEEECCCCCCEEE	52.77	24703693
245	Phosphorylation	IKDGTTRTTSLELFM CCCCCCEEEHHHHHH	20.74	29899451
246	Phosphorylation	KDGTTRTTSLELFMY CCCCCEEEHHHHHHH	28.20	29472430
247	Phosphorylation	DGTTRTTSLELFMYL CCCCEEEHHHHHHHH	21.16	29472430
260	Ubiquitination	YLNEVAGKHGVGRID HHHHHCCCCCCCCEE	27.70	22790023
260	Acetylation	YLNEVAGKHGVGRID HHHHHCCCCCCCCEE	27.70	23864654
277	Ubiquitination	ENRFIGMKSRGIYET CCCCCCCCCCCEEEC	31.61	-
277	Malonylation	ENRFIGMKSRGIYET CCCCCCCCCCCEEEC	31.61	26320211
278	Phosphorylation	NRFIGMKSRGIYETP CCCCCCCCCCEEECC	27.18	-
310	Succinylation	DREVRKIKQGLGLKF CHHHHHHHHCCCCCH	41.15	23954790
310	Ubiquitination	DREVRKIKQGLGLKF CHHHHHHHHCCCCCH	41.15	-
322	Phosphorylation	LKFAELVYTGFWHSP CCHHHHHHCCCCCCH	17.48	23984901
323	Phosphorylation	KFAELVYTGFWHSPE CHHHHHHCCCCCCHH	20.58	23984901
331	S-palmitoylation	GFWHSPECEFVRHCI CCCCCHHHHHHHHHH	5.81	28526873
340	Ubiquitination	FVRHCIQKSQERVEG HHHHHHHHHHHHHCC	33.91	-
340	Acetylation	FVRHCIQKSQERVEG HHHHHHHHHHHHHCC	33.91	23864654
340	Malonylation	FVRHCIQKSQERVEG HHHHHHHHHHHHHCC	33.91	26320211
348	Succinylation	SQERVEGKVQVSVFK HHHHHCCCEEEEEEC	18.91	23954790
348	Malonylation	SQERVEGKVQVSVFK HHHHHCCCEEEEEEC	18.91	26320211
352	Phosphorylation	VEGKVQVSVFKGQVY HCCCEEEEEECCEEE	12.54	22006019
355	Succinylation	KVQVSVFKGQVYILG CEEEEEECCEEEEEC	46.40	23954790
355	Acetylation	KVQVSVFKGQVYILG CEEEEEECCEEEEEC	46.40	23864654
383	Phosphorylation	SMNVQGDYEPIDATG ECCCCCCCCCCCCCC	30.35	-
402	Phosphorylation	NSLRLKEYHRLQSKV CCCCHHHHHHHHHCC	7.22	29514104
408	Malonylation	EYHRLQSKVTAK--- HHHHHHHCCCCC---	30.55	26320211
408	Ubiquitination	EYHRLQSKVTAK--- HHHHHHHCCCCC---	30.55	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
219	S	Phosphorylation	Kinase	MAPK14	P47811	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ASSY_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ASSY_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of ASSY_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.