ARI5B_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ARI5B_MOUSE
• UniProt AC: Q8BM75
• Protein Name: AT-rich interactive domain-containing protein 5B
• Gene Name: Arid5b
• Organism: Mus musculus (Mouse).
• Sequence Length: 1188
• Subcellular Localization: Nucleus .
• Protein Description: Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-337, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver (By similarity). Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation..
• Protein Sequence: MEPNSLQWVGSPCGLHGPYIFYKAFQFHLEGKPRILSLGDFFFVRCTPKDPICIAELQLLWEERTSRQLLSSSKLYFLPEDTPQGRNSDHGEDEVIAVSEKVIVKLEDLVKWAHSDFSKWRCGLRATPVKTEAFGRNGQKEALLRYRQSTLNSGLNFKDVLKEKADLGEDEEETNVIVLSYPQYCRYRSMLKRIQDKPSSILTDQFALALGGIAVVSRNPQILYCRDTFDHPTLIENESVCDEFAPNLKGRPRKKKTCPQRRDSFSGSKDPNNNCDGKVISKVKGEARSALTKPKNNHNNCKKTSNEEKPKLSIGEECRADEQAFLVALYKYMKERKTPIERIPYLGFKQINLWTMFQAAQKLGGYETITARRQWKHIYDELGGNPGSTSAATCTRRHYERLILPYERFIKGEEDKPLPPIKPRKQENNTQENENKTKVSGNKRIKQEMAKNKKEKENTPKPQDTSEVSSEQRKEEETLNHKSAPEPLPAPEVKGKPEGHKDLGARAPVSRADPEKANETDQGSNSEKEAEEMGDKGLAPLLPSPPLPPEKDSAPTPGAGKQPLASPSTQMDSKQEAKPCCFTESPEKDLQGAPFSSFSATKPPLTSQNEAEEEQLPATANYIANCTVKVDQLGSDDIHTALKQTPKVLVVQSFDMFKDKDLTGPMNENHGLNYTPLLYSRGNPGIMSPLAKKKLLSQVSGASLSSSYPYGSPPPLISKKKLIAREDLCSGLSQGHHSQSSDHTAVSRPSVIQHVQSFKNKASEDRKSINDIFKHDKLSRSDAHRCGFSKHQLGSLADSYILKQETQEGKDKLLEKRAVSHAHVPSFLADFYSSPHLHSLYRHTEHHLHNEQSSKYAARDAYQESENGAFLSHKHPEKIHVNYLASLHLQDKKVAAAEASTDDQPTDLSLPKNPHKLTSKVLGLAHSTSGSQEIKGASQFQVVSNQSRDCHPKACRVSPMTMSGPKKYPESLARSGKPHQVRLENFRKMEGMVHPILHRKMSPQNIGAARPIKRSLEDLDLVIAGKKARAVSPLDPAKEASGKEKASEQESEGNKGAYGGHSGAASEGHKLPLSTPIFPGLYSGSLCNSGLNSRLPAGYSHSLQYLKNQTVLSPLMQPLAFHSLVMQRGIFTSPTNSQQLYRHLAAATPVGSSYGDLLHNSIYPLAGINPQAAFPSSQLSSVHPSTKL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
37	Phosphorylation	EGKPRILSLGDFFFV CCCCEEEEECCEEEE	28.12	27841257
131	Phosphorylation	LRATPVKTEAFGRNG EECCCCCCCCCCCCC	32.55	22942356
217	Phosphorylation	LGGIAVVSRNPQILY HCCEEEECCCCEEEE	21.53	-
264	Phosphorylation	TCPQRRDSFSGSKDP CCCCCCCCCCCCCCC	21.35	27087446
266	Phosphorylation	PQRRDSFSGSKDPNN CCCCCCCCCCCCCCC	46.22	25266776
268	Phosphorylation	RRDSFSGSKDPNNNC CCCCCCCCCCCCCCC	32.46	30635358
337	Methylation	YKYMKERKTPIERIP HHHHHHCCCCHHHCC	60.80	-
337	"N6,N6-dimethyllysine"	YKYMKERKTPIERIP HHHHHHCCCCHHHCC	60.80	-
453	Ubiquitination	KQEMAKNKKEKENTP HHHHHHCHHHHCCCC	62.70	-
454	Ubiquitination	QEMAKNKKEKENTPK HHHHHCHHHHCCCCC	81.21	-
459	Phosphorylation	NKKEKENTPKPQDTS CHHHHCCCCCCCCHH	33.51	29550500
465	Phosphorylation	NTPKPQDTSEVSSEQ CCCCCCCHHHCCHHH	22.88	29550500
483	Phosphorylation	EETLNHKSAPEPLPA HHHCCCCCCCCCCCC	41.32	25338131
524	Phosphorylation	ANETDQGSNSEKEAE CCCCCCCCCHHHHHH	31.39	27087446
526	Phosphorylation	ETDQGSNSEKEAEEM CCCCCCCHHHHHHHH	52.31	26824392
544	Phosphorylation	GLAPLLPSPPLPPEK CCCCCCCCCCCCCCC	38.84	30352176
556	Phosphorylation	PEKDSAPTPGAGKQP CCCCCCCCCCCCCCC	33.79	25338131
566	Phosphorylation	AGKQPLASPSTQMDS CCCCCCCCCCCCCCC	27.37	26824392
568	Phosphorylation	KQPLASPSTQMDSKQ CCCCCCCCCCCCCCC	28.86	29472430
569	Phosphorylation	QPLASPSTQMDSKQE CCCCCCCCCCCCCCC	31.15	29472430
583	Phosphorylation	EAKPCCFTESPEKDL CCCCCCCCCCCCCCC	22.95	25619855
585	Phosphorylation	KPCCFTESPEKDLQG CCCCCCCCCCCCCCC	34.99	25619855
688	Phosphorylation	RGNPGIMSPLAKKKL CCCCCCCCHHHHHHH	18.16	26824392
710	Phosphorylation	SLSSSYPYGSPPPLI CCCCCCCCCCCCCCC	23.23	28576409
712	Phosphorylation	SSSYPYGSPPPLISK CCCCCCCCCCCCCCH	28.52	23608596
718	Phosphorylation	GSPPPLISKKKLIAR CCCCCCCCHHHHHHH	46.21	-
781	Phosphorylation	KHDKLSRSDAHRCGF HCCCCCHHHHHHCCC	35.45	-
958	Phosphorylation	HPKACRVSPMTMSGP CCCCCEECCCCCCCC	6.93	22942356
971	Phosphorylation	GPKKYPESLARSGKP CCCCCHHHHHHCCCC	23.87	28059163
1002	Phosphorylation	PILHRKMSPQNIGAA HHHCCCCCHHHCCCC	26.77	26824392
1015	Phosphorylation	AARPIKRSLEDLDLV CCCCCCCCHHHCCEE	30.98	25338131
1032	Phosphorylation	GKKARAVSPLDPAKE CCCEEECCCCCHHHH	20.82	26824392
1133	Phosphorylation	MQRGIFTSPTNSQQL HHCCCCCCCCCHHHH	20.98	-
1135	Phosphorylation	RGIFTSPTNSQQLYR CCCCCCCCCHHHHHH	47.47	22006019

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ARI5B_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
337	K	Methylation	Methylation at Lys-337 prevents DNA-binding.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ARI5B_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of ARI5B_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.