ARI1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ARI1_MOUSE
• UniProt AC: Q9Z1K5
• Protein Name: E3 ubiquitin-protein ligase ARIH1
• Gene Name: Arih1
• Organism: Mus musculus (Mouse).
• Sequence Length: 555
• Subcellular Localization: Cytoplasm . Nucleus . Nucleus, Cajal body . Mainly cytoplasmic. Present in Lewy body.
• Protein Description: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report EIF4E2 ubiquitination leads to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2..
• Protein Sequence: MDSDEGYNYEFDEDEECSEEDSGAEEEEDDDEDEPDDDNLDLGEVELVEPGLGVGGERDGLLCGETGGGGGSALGPGGGGGGGGGGGGPGHEQEEDYRYEVLTAEQILQHMVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
52	Ubiquitination	VELVEPGLGVGGERD EEEECCCCCCCCCCC	8.01	27667366
60	Ubiquitination	GVGGERDGLLCGETG CCCCCCCCEECCCCC	27.11	27667366
140	Ubiquitination	LSHFNWDKEKLMERY HHHCCCCHHHHHHHH	48.65	22790023
140	Acetylation	LSHFNWDKEKLMERY HHHCCCCHHHHHHHH	48.65	23806337
142	Ubiquitination	HFNWDKEKLMERYFD HCCCCHHHHHHHHCC	59.90	27667366
154	Ubiquitination	YFDGNLEKLFAECHV HCCCCHHHHHHHCEE	52.66	22790023
166	Ubiquitination	CHVINPSKKSRTRQM CEECCCCHHHCCCCC	56.90	22790023
314	Ubiquitination	ENWHDPVKCKWLKKW CCCCCHHHHHHHHHH	35.17	22790023
316	Ubiquitination	WHDPVKCKWLKKWIK CCCHHHHHHHHHHHH	50.25	-
324	Ubiquitination	WLKKWIKKCDDDSET HHHHHHHHCCCCCHH	33.49	22790023
347	Ubiquitination	KECPKCHVTIEKDGG CCCCCCEEEEEECCC	8.93	27667366
355	Ubiquitination	TIEKDGGCNHMVCRN EEEECCCCCCEEECC	3.75	27667366
378	Ubiquitination	WVCLGPWEPHGSAWY EEECCCCCCCCCCCC	30.47	27667366
396	Ubiquitination	RYNEDDAKAARDAQE CCCHHHHHHHHHHHH	49.96	-
437	Ubiquitination	HKLYAQVKQKMEEMQ HHHHHHHHHHHHHHH	31.92	27667366
458	Malonylation	IEVQFLKKAVDVLCQ HHHHHHHHHHHHHHH	56.65	26320211
512	Phosphorylation	GYLERDISQDSLQDI HHHHCCCCHHHHHHH	32.02	25293948
515	Phosphorylation	ERDISQDSLQDIKQK HCCCCHHHHHHHHHH	21.94	25293948

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ARI1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ARI1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ARI1_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of ARI1_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.