dbPTM

AMD_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AMD_RAT
UniProt AC	P14925
Protein Name	Peptidyl-glycine alpha-amidating monooxygenase
Gene Name	Pam
Organism	Rattus norvegicus (Rat).
Sequence Length	976
Subcellular Localization	Cytoplasmic vesicle, secretory vesicle membrane Single-pass membrane protein. Secretory granules.
Protein Description	Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity..
Protein Sequence	MAGRARSGLLLLLLGLLALQSSCLAFRSPLSVFKRFKETTRSFSNECLGTIGPVTPLDASDFALDIRMPGVTPKESDTYFCMSMRLPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSVHLTRVPQPLIAGMYLMMSVDTVIPPGEKVVNADISCQYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQNPQLPQAFYPVEHPVDVTFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKYALSFMTCTKNVAPDMFRTIPAEANIPIPVKPDMVMMHGHHKEAENKEKSALMQQPKQGEEEVLEQGDFYSLLSKLLGEREDVHVHKYNPTEKTESGSDLVAEIANVVQKKDLGRSDAREGAEHEEWGNAILVRDRIHRFHQLESTLRPAESRAFSFQQPGEGPWEPEPSGDFHVEEELDWPGVYLLPGQVSGVALDSKNNLVIFHRGDHVWDGNSFDSKFVYQQRGLGPIEEDTILVIDPNNAEILQSSGKNLFYLPHGLSIDTDGNYWVTDVALHQVFKLDPHSKEGPLLILGRSMQPGSDQNHFCQPTDVAVEPSTGAVFVSDGYCNSRIVQFSPSGKFVTQWGEESSGSSPRPGQFSVPHSLALVPHLDQLCVADRENGRIQCFKTDTKEFVREIKHASFGRNVFAISYIPGFLFAVNGKPYFGDQEPVQGFVMNFSSGEIIDVFKPVRKHFDMPHDIVASEDGTVYIGDAHTNTVWKFTLTEKMEHRSVKKAGIEVQEIKEAEAVVEPKVENKPTSSELQKMQEKQKLSTEPGSGVSVVLITTLLVIPVLVLLAIVMFIRWKKSRAFGDHDRKLESSSGRVLGRFRGKGSGGLNLGNFFASRKGYSRKGFDRVSTEGSDQEKDEDDGTESEEEYSAPLPKPAPSS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
765	N-linked_Glycosylation	PVQGFVMNFSSGEII CCCEEEEECCCCCEE	28.48	2211657
774 (in isoform 3)	Sulfation	-	4.71	-
792 (in isoform 4)	Sulfation	-	51.20	-
814	Acetylation	WKFTLTEKMEHRSVK EEEEECHHHCCCCHH	44.33	22902405
846	Phosphorylation	PKVENKPTSSELQKM CCCCCCCCHHHHHHH	46.63	28432305
847	Phosphorylation	KVENKPTSSELQKMQ CCCCCCCHHHHHHHH	30.27	28432305
848	Phosphorylation	VENKPTSSELQKMQE CCCCCCHHHHHHHHH	44.82	28432305
904	Ubiquitination	AFGDHDRKLESSSGR CCCCCCCCCCCCCCC	63.76	-
921	Phosphorylation	GRFRGKGSGGLNLGN ECCCCCCCCCCCCHH	32.92	27097102
932	Phosphorylation	NLGNFFASRKGYSRK CCHHHHHCCCCCCCC	29.39	27097102
937	Phosphorylation	FASRKGYSRKGFDRV HHCCCCCCCCCCCCC	35.86	10409666
945	Phosphorylation	RKGFDRVSTEGSDQE CCCCCCCCCCCCCCC	22.95	22673903
946	Phosphorylation	KGFDRVSTEGSDQEK CCCCCCCCCCCCCCC	41.32	20374556
949	Phosphorylation	DRVSTEGSDQEKDED CCCCCCCCCCCCCCC	29.86	10574929
959	Phosphorylation	EKDEDDGTESEEEYS CCCCCCCCCCHHHCC	43.25	22673903
961	Phosphorylation	DEDDGTESEEEYSAP CCCCCCCCHHHCCCC	50.58	22673903
965	Sulfation	GTESEEEYSAPLPKP CCCCHHHCCCCCCCC	17.26	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
937	S	Phosphorylation	Kinase	PRKCA	P05696	GPS
946	T	Phosphorylation	Kinase	CSNK2A1	P19139	GPS
949	S	Phosphorylation	Kinase	CSNK2A1	P19139	GPS
949	S	Phosphorylation	Kinase	UHMK1	Q63285	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AMD_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AMD_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
UBC_HUMAN	UBC	physical	16098968

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AMD_RAT

Related Literatures of Post-Translational Modification

N-linked Glycosylation
Reference	PubMed
"Purification and characterization of functional recombinant alpha-amidating enzyme secreted from mammalian cells."; Beaudry G.A., Mehta N.M., Ray M.L., Bertelsen A.H.; J. Biol. Chem. 265:17694-17699(1990). Cited for: PROTEIN SEQUENCE OF 26-42, AND GLYCOSYLATION AT ASN-765.	2211657 [Abstract]