dbPTM

ALAT1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ALAT1_HUMAN
UniProt AC	P24298
Protein Name	Alanine aminotransferase 1
Gene Name	GPT
Organism	Homo sapiens (Human).
Sequence Length	496
Subcellular Localization	Cytoplasm.
Protein Description	Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity)..
Protein Sequence	MASSTGDRSQAVRHGLRAKVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFLRQVLALCVNPDLLSSPNFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDVARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFHAKFTLEYS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MASSTGDRS ------CCCCCCCHH	16.52	1931970
22	Phosphorylation	GLRAKVLTLDGMNPR CHHEEEEEECCCCHH	26.28	28857561
35	Phosphorylation	PRVRRVEYAVRGPIV HHHHEHHHHHHCHHH	13.92	21253578
61	Phosphorylation	QGVKKPFTEVIRANI HCCCCCHHHHHHHHC	37.56	21406692
178	Phosphorylation	LVAGEGHTRTGVLIP HHCCCCCCCCEEEEE	40.56	26074081
180	Phosphorylation	AGEGHTRTGVLIPIP CCCCCCCCEEEEECC	32.56	26074081
189	Phosphorylation	VLIPIPQYPLYSATL EEEECCCCCCCCCHH	7.00	26074081
246	Phosphorylation	VINPGNPTGQVQTRE EECCCCCCCCCCHHH	44.22	28787133
251	Phosphorylation	NPTGQVQTRECIEAV CCCCCCCHHHHHHHH	29.58	29978859
290	Acetylation	GSQFHSFKKVLMEMG CCCHHHHHHHHHHHC	45.01	24886787
300	Phosphorylation	LMEMGPPYAGQQELA HHHHCCCCCCHHHHH	26.88	20068231
308	Phosphorylation	AGQQELASFHSTSKG CCHHHHHHHCCCCCC	35.80	20068231
311	Phosphorylation	QELASFHSTSKGYMG HHHHHHCCCCCCCCC	32.16	20068231
312	Phosphorylation	ELASFHSTSKGYMGE HHHHHCCCCCCCCCC	26.36	20068231
313	Phosphorylation	LASFHSTSKGYMGEC HHHHCCCCCCCCCCC	27.39	20068231
314	Other	ASFHSTSKGYMGECG HHHCCCCCCCCCCCC	54.90	-
314	N6-(pyridoxal phosphate)lysine	ASFHSTSKGYMGECG HHHCCCCCCCCCCCC	54.90	-
316	Phosphorylation	FHSTSKGYMGECGFR HCCCCCCCCCCCCCC	12.99	20068231
413	Phosphorylation	NPVQGAMYSFPRVQL CCCCCCCCCCCCCCC	13.46	28857561
414	Phosphorylation	PVQGAMYSFPRVQLP CCCCCCCCCCCCCCC	18.71	-
464	Phosphorylation	FGQREGTYHFRMTIL CCCCCCEEEEEEEEC	15.15	26437602
469	Phosphorylation	GTYHFRMTILPPLEK CEEEEEEEECCHHHH	18.33	22210691
485	Phosphorylation	RLLLEKLSRFHAKFT HHHHHHHHHHHHHEE	43.92	20068231
496	Phosphorylation	AKFTLEYS------- HHEEEECC-------	25.11	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of ALAT1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ALAT1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
2	Acetylation	11 (9)	A ⇒ E;V	rs139849083	GLP-1 levels in response to oral glucose tolerance test (fasting)	29093273

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
COF1_HUMAN	CFL1	physical	26344197

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
DB00160	L-Alanine
DB00780	Phenelzine

Regulatory Network of ALAT1_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Complete amino acid sequence of human liver cytosolic alanineaminotransferase (GPT) determined by a combination of conventional andmass spectral methods."; Ishiguro M., Takio K., Suzuki M., Oyama R., Matsuzawa T., Titani K.; Biochemistry 30:10451-10457(1991). Cited for: PROTEIN SEQUENCE OF 2-496.	1931970 [Abstract]