dbPTM

ALA2_ARATH - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ALA2_ARATH
UniProt AC	P98205
Protein Name	Phospholipid-transporting ATPase 2 {ECO:0000303\|PubMed:11402198}
Gene Name	ALA2 {ECO:0000303\|PubMed:11402198}
Organism	Arabidopsis thaliana (Mouse-ear cress).
Sequence Length	1107
Subcellular Localization	Endoplasmic reticulum membrane Multi-pass membrane protein . Prevacuolar compartment membrane Multi-pass membrane protein . Requires the presence of an ALIS protein to exit the endoplasmic reticulum to the prevacuolar compartment.
Protein Description	Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Requires an interaction with a protein of the ALIS family for activity. Specific for phosphatidylserine and has no activity with lysolipid, phosphatidylcholine or phosphatidylethanolamine..
Protein Sequence	MKRFVYINDDEASKELCCDNRISNRKYTLWNFLPKNLWEQFSRFMNQYFLLIACLQLWSLITPVNPASTWGPLIFIFAVSASKEAWDDYHRYLSDKKANEKEVWIVKQGIKKHIQAQDIQVGNIVWLRENDEVPCDLVLLGTSDPQGVCYVETAALDGETDLKTRVIPSACVGIDLELLHKMKGVIECPVPDKDIRRFDANMRLFPPFIDNDVCSLTIKNTLLQSCYLRNTEWACGVSVYTGNQTKLGMSRGIAEPKLTAMDAMIDKLTGAIFVFQIVVVLVLGIAGNVWKDTEARKQWYVQYPEEAPWYELLVIPLRFELLCSIMIPISIKVSLDLVKGLYAKFIEWDVEMIDQETGTASYAANTAISEDLGQVEYILTDKTGTLTDNKMIFRRCCIGGIFYGNENGDALKDAQLLNAITSGSTDVIRFLTVMAICNTVLPVQSKAGDIVYKAQSQDEDALVIAASKLHMVFVGKNANLLEIRFNGSVIRYEVLEILEFTSDRKRMSVVVKDCQNGKIILLSKGADEAILPYARAGQQTRTIGDAVEHYSQLGLRTLCLAWRELEENEYLEWSVKFKEASSLLVDREWRIAEVCQRLEHDLYILGVTAIEDRLQDGVPETIETLRKAGINFWMLTGDKQNTAIQIALSCNFISPEPKGQLLMIDGKTEEDVSRSLERVLLTMRITASEPKDVAFVIDGWALEIALKHHRKDFVELAILSRTAICCRVTPSQKAQLVEILKSCDYRTLAIGDGGNDVRMIQQADIGVGISGREGLQAARAADYSIGRFRFLKRLILVHGRYSYNRTAFLSQYSFYKSLLICFIQIFFSFISGVSGTSLFNSVSLMAYNVFYTSVPVLVSVIDKDLSEASVMQHPQILFYCQAGRLLNPSTFAGWFGRSLFHAIIVFVITIHAYAYEKSEMEELGMVALSGCIWLQAFVVAQETNSFTVLQHLSIWGNLVGFYAINFLFSAIPSSGMYTIMFRLCSQPSYWITMFLIVGAGMGPIFALKYFRYTYRPSKINILQQAERMGGPILTLGNIETQPRTIEKDLSPISITQPKNRSPVYEPLLSDSPNATRRSFGPGTPFEFFQSQSRLSSSSGYTRNCKDN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
28	Phosphorylation	RISNRKYTLWNFLPK CCCCCCCCHHHCCCH	28.47	19880383
259	Phosphorylation	GIAEPKLTAMDAMID CCCCCCHHHHHHHHH	26.51	24894044
608	Phosphorylation	DLYILGVTAIEDRLQ CEEEEEEEHHHHHHH	21.81	26811356
624	Phosphorylation	GVPETIETLRKAGIN CCCHHHHHHHHHCCC	28.19	26811356
1050	Phosphorylation	RTIEKDLSPISITQP CCCCCCCCCCCCCCC	30.26	19245862
1061	Phosphorylation	ITQPKNRSPVYEPLL CCCCCCCCCCCCCHH	28.28	19376835
1064	Phosphorylation	PKNRSPVYEPLLSDS CCCCCCCCCCHHCCC	18.40	19376835
1069	Phosphorylation	PVYEPLLSDSPNATR CCCCCHHCCCCCCCC	43.66	19376835
1071	Phosphorylation	YEPLLSDSPNATRRS CCCHHCCCCCCCCCC	19.37	30291188
1075	Phosphorylation	LSDSPNATRRSFGPG HCCCCCCCCCCCCCC	33.00	19376835
1078	Phosphorylation	SPNATRRSFGPGTPF CCCCCCCCCCCCCCH	31.12	19880383
1083	Phosphorylation	RRSFGPGTPFEFFQS CCCCCCCCCHHHHHC	28.12	30407730
1090	Phosphorylation	TPFEFFQSQSRLSSS CCHHHHHCCHHHCCC	25.60	30407730
1092	Phosphorylation	FEFFQSQSRLSSSSG HHHHHCCHHHCCCCC	40.20	30407730
1095	Phosphorylation	FQSQSRLSSSSGYTR HHCCHHHCCCCCCCC	27.36	25561503
1096	Phosphorylation	QSQSRLSSSSGYTRN HCCHHHCCCCCCCCC	32.87	25561503
1097	Phosphorylation	SQSRLSSSSGYTRNC CCHHHCCCCCCCCCC	25.14	29654922

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of ALA2_ARATH !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ALA2_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ALA2_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of ALA2_ARATH !!

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ALA2_ARATH

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphoproteomic analysis of nuclei-enriched fractions fromArabidopsis thaliana."; Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A.,Andreasson E., Rathjen J.P., Peck S.C.; J. Proteomics 72:439-451(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1050, AND MASSSPECTROMETRY.	19245862 [Abstract]