AIFM1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AIFM1_RAT
• UniProt AC: Q9JM53
• Protein Name: Apoptosis-inducing factor 1, mitochondrial
• Gene Name: Aifm1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 612
• Subcellular Localization: Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of a
• Protein Description: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis,and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity)..
• Protein Sequence: MFRCGGLAGAFKQKLVPLVRSVCVQRPKQRNRLPGNLFQQWRVPLELQMARQMASSGPSGGKMDNSVLVLIVGLSTIGAGAYAYKTIKEDQKRYNERIMGLGLSPEEKQRRAIASAAEGGSVPPIRVPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLQFRQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVHLDVRGNMVKLNDGSQITFEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRALEKISREVKSITVIGGGFLGSELACALGRKSQASGIEVIQLFPEKGNMGKILPEYLSNWTMEKVKREGVKVMPNAIVQSVGVSGGKLLIKLKDGRKVETDHIVTAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSDPAVPQVPVEGEDYGKGVIFYLRDKVVVGIVLWNVFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
104	Phosphorylation	RIMGLGLSPEEKQRR HHCCCCCCHHHHHHH	28.59	23984901
108	Succinylation	LGLSPEEKQRRAIAS CCCCHHHHHHHHHHH	47.66	-
108	Succinylation	LGLSPEEKQRRAIAS CCCCHHHHHHHHHHH	47.66	-
115	Phosphorylation	KQRRAIASAAEGGSV HHHHHHHHHHCCCCC	23.15	30181290
121	Phosphorylation	ASAAEGGSVPPIRVP HHHHCCCCCCCCCCC	42.16	30181290
198	Acetylation	QFRQWNGKERSIYFQ EEEECCCCCCEEEEC	46.90	22902405
231	Acetylation	VAVLTGKKVVHLDVR EEEEECCEEEEEECC	51.32	22902405
243	Acetylation	DVRGNMVKLNDGSQI ECCCCEEECCCCCEE	30.70	22902405
259	Phosphorylation	FEKCLIATGGTPRSL EEEEEEECCCCCCCH	29.20	23991683
262	Phosphorylation	CLIATGGTPRSLSAI EEEECCCCCCCHHHH	19.51	23991683
265	Phosphorylation	ATGGTPRSLSAIDRA ECCCCCCCHHHHHHC	28.07	23984901
267	Phosphorylation	GGTPRSLSAIDRAGA CCCCCCHHHHHHCCC	25.15	29779826
294	Acetylation	GDFRALEKISREVKS CCHHHHHHHCHHCCE	47.00	29557419
336	Acetylation	VIQLFPEKGNMGKIL EEECCCCCCCHHHCH	56.68	22902405
341	Acetylation	PEKGNMGKILPEYLS CCCCCHHHCHHHHHH	30.24	22902405
370	Phosphorylation	MPNAIVQSVGVSGGK CCCEEEEEECCCCCE	15.22	30181290
374	Phosphorylation	IVQSVGVSGGKLLIK EEEEECCCCCEEEEE	35.58	30181290
381	Acetylation	SGGKLLIKLKDGRKV CCCEEEEEECCCCEE	49.83	72618231
387	Acetylation	IKLKDGRKVETDHIV EEECCCCEEECCEEE	50.37	22902405
415	Phosphorylation	TGGLEIDSDFGGFRV HCCEEECCCCCCEEE	40.27	30181290
520	Phosphorylation	QDNPKSATEQSGTGI CCCCCCHHHCCCCCC	41.47	-
523	Phosphorylation	PKSATEQSGTGIRSE CCCHHHCCCCCCCCC	31.90	23991683
525	Phosphorylation	SATEQSGTGIRSESE CHHHCCCCCCCCCCC	34.38	23991683
529	Phosphorylation	QSGTGIRSESETESE CCCCCCCCCCCCCCC	43.48	-
589	Acetylation	NRMPIARKIIKDGEQ CCCHHHHHHHCCCCC	39.50	22902405
592	Acetylation	PIARKIIKDGEQHED HHHHHHHCCCCCCCC	64.25	22902405

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of AIFM1_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
254	K	ubiquitylation	Ubiquitination at Lys-254 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AIFM1_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of AIFM1_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.