AFAD_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AFAD_RAT
• UniProt AC: O35889
• Protein Name: Afadin
• Gene Name: Afdn {ECO:0000312|RGD:708561}
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1829
• Subcellular Localization: Cell junction, adherens junction . Not found at cell-matrix AJs.
• Protein Description: Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm..
• Protein Sequence: MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEGKPLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQFGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDIHSGTALPASRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQDYRRRESRTQDAAGPELMLPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENTELTQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSPYTSGTAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPVQNQWPNYEEKPHMHTESDHASIAIQRVTRSQEELREEKVYQLERHRVESGMDRKCDSDMWINQSSSVESSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPAHYTSDFDGISMDLPLPPPPANQAAPQSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHELQNKGDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREVEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRLLEPEEPGLSRPPLPQDYEPPSQSSAPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDDDDEEENYVPAGPNSYSGSAGTTAGTYDAPRDTREKLSRSQDADLPGSSGAPENLTFRERQRLFSQGQDVSDKVKASRKLTELENELNTK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
181	Phosphorylation	KEALRQASDKEERPS HHHHHHCCCCCCCCC	40.66	25403869
203	Phosphorylation	SRLAAEVYKDMPETS HHHHHHHHCCCCCCC	7.87	22276854
214	Phosphorylation	PETSFTRTISNPEVV CCCCCEECCCCHHHH	26.21	27097102
216	Phosphorylation	TSFTRTISNPEVVMK CCCEECCCCHHHHHH	45.80	27097102
239	Phosphorylation	KRMQEFRSSDGRPDS HHHHHHHCCCCCCCC	37.79	28432305
240	Phosphorylation	RMQEFRSSDGRPDSG HHHHHHCCCCCCCCC	39.57	28432305
246	Phosphorylation	SSDGRPDSGGTLRIY CCCCCCCCCCEEEEE	41.63	28432305
249	Phosphorylation	GRPDSGGTLRIYADS CCCCCCCEEEEEECC	19.18	28432305
256	Phosphorylation	TLRIYADSLKPNIPY EEEEEECCCCCCCCC	30.07	-
336	Acetylation	FREWPSDKGILVFQL HHHCCCCCCEEEEEE	52.79	22902405
362	Acetylation	MKKHVEGKPLKGKDR HHHCCCCCCCCCCCC	33.40	22902405
373	Phosphorylation	GKDRADGSGYGSALP CCCCCCCCCCCCCCC	29.41	28432305
391	Phosphorylation	LPYLVELSPGRRNHF CCEEEEECCCCCCCC	16.29	27097102
424	Phosphorylation	KLYRLQLSVTEVGTE CEEEEEEEEEECCCC	17.65	22108457
426	Phosphorylation	YRLQLSVTEVGTEKF EEEEEEEEECCCCCC	22.90	28432305
430	Phosphorylation	LSVTEVGTEKFDDNS EEEEECCCCCCCCCC	40.97	23984901
509	Phosphorylation	PIQDHVLSKRSVDGG HHHCCCCCCCCCCCC	25.57	25575281
512	Phosphorylation	DHVLSKRSVDGGLMV CCCCCCCCCCCCEEE	28.44	27097102
551	Phosphorylation	TALPASRSTTRLDSD CCCCCCCCCCCCCCC	31.47	23984901
552	Phosphorylation	ALPASRSTTRLDSDR CCCCCCCCCCCCCCC	18.15	23984901
553	Phosphorylation	LPASRSTTRLDSDRV CCCCCCCCCCCCCCC	30.40	23984901
557	Phosphorylation	RSTTRLDSDRVSSAS CCCCCCCCCCCCCCC	31.72	22108457
561	Phosphorylation	RLDSDRVSSASSTAE CCCCCCCCCCCHHHH	22.53	28432305
562	Phosphorylation	LDSDRVSSASSTAER CCCCCCCCCCHHHHC	29.34	18779572
564	Phosphorylation	SDRVSSASSTAERGM CCCCCCCCHHHHCCC	30.12	28432305
565	Phosphorylation	DRVSSASSTAERGMV CCCCCCCHHHHCCCC	31.21	28432305
566	Phosphorylation	RVSSASSTAERGMVK CCCCCCHHHHCCCCC	30.00	28432305
589	Phosphorylation	QDYRRRESRTQDAAG HHHHHHHHHCCCCCC	38.52	4298673
610	Phosphorylation	ASIEFRESSEDSFLS CEEEEECCCCCCHHH	34.47	28689409
611	Phosphorylation	SIEFRESSEDSFLSA EEEEECCCCCCHHHH	40.44	28689409
614	Phosphorylation	FRESSEDSFLSAIIN EECCCCCCHHHHHHH	24.84	28689409
625	Phosphorylation	AIINYTNSSTVHFKL HHHHCCCCCCEEEEE	21.42	28689409
626	Phosphorylation	IINYTNSSTVHFKLS HHHCCCCCCEEEEEC	36.20	28689409
647	Phosphorylation	MACRYVLSSQHRPDI HHHHHHHHCCCCCCC	20.22	25575281
648	Phosphorylation	ACRYVLSSQHRPDIS HHHHHHHCCCCCCCC	26.33	27097102
655	Phosphorylation	SQHRPDISPTERTHK CCCCCCCCCCHHHHH	32.90	27097102
657	Phosphorylation	HRPDISPTERTHKAI CCCCCCCCHHHHHHH	31.17	27097102
993	Phosphorylation	IYFEGADYESHLMRE EEECCCCHHHHHHHC	20.85	21940666
1088	Phosphorylation	AAELMTRTSSVVTLE HHHHHHHCCCEEEHH	19.04	22108457
1089	Phosphorylation	AELMTRTSSVVTLEV HHHHHHCCCEEEHHH	20.18	21738781
1090	Phosphorylation	ELMTRTSSVVTLEVA HHHHHCCCEEEHHHH	21.72	23712012
1093	Phosphorylation	TRTSSVVTLEVAKQG HHCCCEEEHHHHHCC	18.29	28689409
1103	Phosphorylation	VAKQGAIYHGLATLL HHHCCHHHHHHHHHH	6.84	24945867
1108	Phosphorylation	AIYHGLATLLNQPSP HHHHHHHHHHCCCCH	36.50	28689409
1114	Phosphorylation	ATLLNQPSPMMQRIS HHHHCCCCHHHHHHH	18.70	28689409
1133	Phosphorylation	SGKPRPKSEGFELYN CCCCCCCCCCCCCCC	45.71	-
1139	Phosphorylation	KSEGFELYNNSAQNG CCCCCCCCCCCCCCC	12.71	28689409
1147	Phosphorylation	NNSAQNGSPESPQMP CCCCCCCCCCCCCCC	32.80	22673903
1150	Phosphorylation	AQNGSPESPQMPWTE CCCCCCCCCCCCCCC	24.66	22108457
1179	Phosphorylation	KNRADHRSSPNVANQ HCCCCCCCCCCCCCC	46.88	28689409
1180	Phosphorylation	NRADHRSSPNVANQP CCCCCCCCCCCCCCC	22.05	29027
1189	Phosphorylation	NVANQPPSPGGKSPY CCCCCCCCCCCCCCC	42.74	23712012
1194	Phosphorylation	PPSPGGKSPYTSGTA CCCCCCCCCCCCCCC	26.81	23984901
1196	Phosphorylation	SPGGKSPYTSGTAAK CCCCCCCCCCCCCEE	22.19	23984901
1197	Phosphorylation	PGGKSPYTSGTAAKI CCCCCCCCCCCCEEE	24.86	23984901
1198	Phosphorylation	GGKSPYTSGTAAKIT CCCCCCCCCCCEEEE	28.44	23984901
1200	Phosphorylation	KSPYTSGTAAKITSV CCCCCCCCCEEEEEE	24.18	27097102
1205	Phosphorylation	SGTAAKITSVSTGNL CCCCEEEEEEECCCC	23.51	27097102
1206	Phosphorylation	GTAAKITSVSTGNLC CCCEEEEEEECCCCC	19.97	27097102
1208	Phosphorylation	AAKITSVSTGNLCTE CEEEEEEECCCCCCC	30.29	29779826
1209	Phosphorylation	AKITSVSTGNLCTEE EEEEEEECCCCCCCC	28.12	27097102
1214	Phosphorylation	VSTGNLCTEEQTPPP EECCCCCCCCCCCCC	45.80	27097102
1218	Phosphorylation	NLCTEEQTPPPRPEA CCCCCCCCCCCCCCC	40.83	27097102
1226	Phosphorylation	PPPRPEAYPIPTQTY CCCCCCCCCCCCCCE	10.47	27097102
1230	Phosphorylation	PEAYPIPTQTYTREY CCCCCCCCCCEEEEE	33.97	27097102
1232	Phosphorylation	AYPIPTQTYTREYFT CCCCCCCCEEEEEEE	29.37	27097102
1233	Phosphorylation	YPIPTQTYTREYFTF CCCCCCCEEEEEEEE	8.25	27097102
1234	Phosphorylation	PIPTQTYTREYFTFP CCCCCCEEEEEEEEC	22.03	27097102
1237	Phosphorylation	TQTYTREYFTFPASK CCCEEEEEEEECCCC	12.48	22108457
1239	Phosphorylation	TYTREYFTFPASKSQ CEEEEEEEECCCCCC	28.01	28432305
1243	Phosphorylation	EYFTFPASKSQDRMA EEEEECCCCCCCCCC	32.83	28432305
1244	Acetylation	YFTFPASKSQDRMAP EEEECCCCCCCCCCC	55.00	22902405
1245	Phosphorylation	FTFPASKSQDRMAPV EEECCCCCCCCCCCC	34.51	28432305
1280	Phosphorylation	SIAIQRVTRSQEELR HHHHHHHHCCHHHHH	27.13	26437020
1282	Phosphorylation	AIQRVTRSQEELREE HHHHHHCCHHHHHHH	32.36	23712012
1292	Phosphorylation	ELREEKVYQLERHRV HHHHHHCHHHHHHHH	20.60	92789
1309	Phosphorylation	GMDRKCDSDMWINQS CCCCCCCCCCEECCC	38.54	25575281
1316	Phosphorylation	SDMWINQSSSVESST CCCEECCCCCCCCCC	21.79	27097102
1317	Phosphorylation	DMWINQSSSVESSTS CCEECCCCCCCCCCC	28.74	23984901
1318	Phosphorylation	MWINQSSSVESSTSS CEECCCCCCCCCCCC	34.84	23984901
1321	Phosphorylation	NQSSSVESSTSSQEH CCCCCCCCCCCCHHH	36.44	23984901
1322	Phosphorylation	QSSSVESSTSSQEHL CCCCCCCCCCCHHHH	20.85	23984901
1323	Phosphorylation	SSSVESSTSSQEHLN CCCCCCCCCCHHHHC	41.50	23984901
1324	Phosphorylation	SSVESSTSSQEHLNH CCCCCCCCCHHHHCC	31.78	23984901
1325	Phosphorylation	SVESSTSSQEHLNHS CCCCCCCCHHHHCCC	39.41	23984901
1332	Phosphorylation	SQEHLNHSSKSVTPA CHHHHCCCCCCCCCC	38.09	27097102
1333	Phosphorylation	QEHLNHSSKSVTPAS HHHHCCCCCCCCCCH	22.77	23984901
1335	Phosphorylation	HLNHSSKSVTPASTL HHCCCCCCCCCCHHC	32.92	18779572
1337	Phosphorylation	NHSSKSVTPASTLTK CCCCCCCCCCHHCCC	21.89	27097102
1340	Phosphorylation	SKSVTPASTLTKSGP CCCCCCCHHCCCCCC	25.75	28432305
1341	Phosphorylation	KSVTPASTLTKSGPG CCCCCCHHCCCCCCC	40.32	16641100
1343	Phosphorylation	VTPASTLTKSGPGRW CCCCHHCCCCCCCCC	24.21	28432305
1459	Phosphorylation	TQSLNPASFSPLATQ HCCCCHHHCCCCCCC	27.75	30181290
1461	Phosphorylation	SLNPASFSPLATQAK CCCHHHCCCCCCCCC	19.26	30181290
1465	Phosphorylation	ASFSPLATQAKPEKP HHCCCCCCCCCCCCC	36.42	30181290
1502	Phosphorylation	IERRDLQYITISKEE CCHHCCEEEEEEHHH	13.82	27097102
1504	Phosphorylation	RRDLQYITISKEELS HHCCEEEEEEHHHHC	17.79	27097102
1506	Phosphorylation	DLQYITISKEELSSG CCEEEEEEHHHHCCC	25.93	22673903
1511	Phosphorylation	TISKEELSSGDSLSP EEEHHHHCCCCCCCC	35.24	25575281
1512	Phosphorylation	ISKEELSSGDSLSPD EEHHHHCCCCCCCCC	60.28	28432305
1515	Phosphorylation	EELSSGDSLSPDPWK HHHCCCCCCCCCHHH	33.98	27097102
1517	Phosphorylation	LSSGDSLSPDPWKRD HCCCCCCCCCHHHHH	31.07	27097102
1578	Phosphorylation	FQKRLQESKQKDEDD HHHHHHHHHHCCCCC	27.68	18779572
1701	Phosphorylation	PQDYEPPSQSSAPSA CCCCCCCCCCCCCCC	54.10	-
1718	Phosphorylation	PPPQRNASYLKTQVL CCCCCCHHHEEEEEC	34.25	29779826
1726	Phosphorylation	YLKTQVLSPDSLFTA HEEEEECCHHHCEEE	28.01	23712012
1729	Phosphorylation	TQVLSPDSLFTAKFV EEECCHHHCEEEEEE	28.85	23984901
1732	Phosphorylation	LSPDSLFTAKFVAYD CCHHHCEEEEEEEEC	33.94	28689409
1777	Phosphorylation	RDTREKLSRSQDADL HHHHHHHHHHCCCCC	40.74	27097102
1779	Phosphorylation	TREKLSRSQDADLPG HHHHHHHHCCCCCCC	29.09	23589303
1787	Phosphorylation	QDADLPGSSGAPENL CCCCCCCCCCCCCCC	24.53	28432305
1788	Phosphorylation	DADLPGSSGAPENLT CCCCCCCCCCCCCCC	44.91	28432305
1795	Phosphorylation	SGAPENLTFRERQRL CCCCCCCCHHHHHHH	32.03	27097102
1804	Phosphorylation	RERQRLFSQGQDVSD HHHHHHHHCCCCHHH	37.68	28689409
1810	Phosphorylation	FSQGQDVSDKVKASR HHCCCCHHHHHHHHH	39.75	28689409
1812	Acetylation	QGQDVSDKVKASRKL CCCCHHHHHHHHHHH	37.82	22902405
1820	Phosphorylation	VKASRKLTELENELN HHHHHHHHHHHHHHC	41.37	23984901

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of AFAD_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AFAD_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AFAD_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of AFAD_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Quantitative phosphoproteomics of vasopressin-sensitive renal cells:regulation of aquaporin-2 phosphorylation at two sites.";
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.;
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557; SER-1179; SER-1180;SER-1189; THR-1337 AND THR-1341, AND MASS SPECTROMETRY.
PubMed: 16641100

"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.";
Moser K., White F.M.;
J. Proteome Res. 5:98-104(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1282, AND MASSSPECTROMETRY.
PubMed: 16396499