ACTH_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ACTH_RAT
• UniProt AC: P63269
• Protein Name: Actin, gamma-enteric smooth muscle
• Gene Name: Actg2
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 376
• Subcellular Localization: Cytoplasm, cytoskeleton.
• Protein Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells..
• Protein Sequence: MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MCEEETTAL ------CCCCCCEEE	10.16	-
3	Acetylation	-----MCEEETTALV -----CCCCCCEEEE	58.53	-
15	Phosphorylation	ALVCDNGSGLCKAGF EEEEECCCCCCCCCC	34.54	21630457
34	Phosphorylation	APRAVFPSIVGRPRH CCCCCCHHHCCCCCC	20.59	5130775
45	Oxidation	RPRHQGVMVGMGQKD CCCCCCEEEECCCCC	2.49	-
48	Oxidation	HQGVMVGMGQKDSYV CCCEEEECCCCCCCC	3.32	-
51	Acetylation	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	7213973
51	Ubiquitination	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	-
53	Phosphorylation	VGMGQKDSYVGDEAQ EECCCCCCCCCCHHH	28.84	25403869
54	Phosphorylation	GMGQKDSYVGDEAQS ECCCCCCCCCCHHHC	20.70	4817623
61	Phosphorylation	YVGDEAQSKRGILTL CCCCHHHCCCCEEEE	31.22	29779826
62	Ubiquitination	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	-
62	Acetylation	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	7215587
67	Phosphorylation	QSKRGILTLKYPIEH HCCCCEEEEECEECC	21.20	28826663
69	Acetylation	KRGILTLKYPIEHGI CCCEEEEECEECCCC	43.69	8269477
70	Nitration	RGILTLKYPIEHGII CCEEEEECEECCCCC	16.74	-
70	Phosphorylation	RGILTLKYPIEHGII CCEEEEECEECCCCC	16.74	22673903
74	Methylation	TLKYPIEHGIITNWD EEECEECCCCCCCHH	33.21	-
78	Phosphorylation	PIEHGIITNWDDMEK EECCCCCCCHHHHHH	28.47	36748793
85	Acetylation	TNWDDMEKIWHHSFY CCHHHHHHHHHHHHH	44.45	7213983
114	Acetylation	TEAPLNPKANREKMT CCCCCCCCCCHHHHH	58.16	30595223
127	Phosphorylation	MTQIMFETFNVPAMY HHHHHHHHCCHHHHH	14.83	21630457
134	Phosphorylation	TFNVPAMYVAIQAVL HCCHHHHHHHHHHHH	6.71	21630457
149	Phosphorylation	SLYASGRTTGIVLDS HHHHCCCCEEEEEEC	32.66	22673903
150	Phosphorylation	LYASGRTTGIVLDSG HHHCCCCEEEEEECC	24.45	22673903
156	Phosphorylation	TTGIVLDSGDGVTHN CEEEEEECCCCCCCC	34.32	-
167	Phosphorylation	VTHNVPIYEGYALPH CCCCCCEECCCCHHH	9.38	3840507
170	Phosphorylation	NVPIYEGYALPHAIM CCCEECCCCHHHHHH	7.94	15569651
187	Phosphorylation	DLAGRDLTDYLMKIL HCCCCCHHHHHHHHH	27.21	22673903
189	Phosphorylation	AGRDLTDYLMKILTE CCCCHHHHHHHHHHH	11.94	18876225
192	Ubiquitination	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	-
192	Acetylation	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	22646709
199	Phosphorylation	KILTERGYSFVTTAE HHHHHCCCCCEEHHC	12.73	174795
200	Phosphorylation	ILTERGYSFVTTAER HHHHCCCCCEEHHCH	18.96	5130783
203	Phosphorylation	ERGYSFVTTAEREIV HCCCCCEEHHCHHHH	20.48	22673903
204	Phosphorylation	RGYSFVTTAEREIVR CCCCCEEHHCHHHHH	22.63	9548407
214	Acetylation	REIVRDIKEKLCYVA HHHHHHHHHHHCEEE	53.75	11921237
219	Phosphorylation	DIKEKLCYVALDFEN HHHHHHCEEEEECCH	10.13	174797
233	Phosphorylation	NEMATAASSSSLEKS HHHHHHCCCCCCCHH	28.43	28551015
234	Phosphorylation	EMATAASSSSLEKSY HHHHHCCCCCCCHHE	21.15	28551015
235	Phosphorylation	MATAASSSSLEKSYE HHHHCCCCCCCHHEE	36.13	28551015
236	Phosphorylation	ATAASSSSLEKSYEL HHHCCCCCCCHHEEC	42.36	28551015
240	Phosphorylation	SSSSLEKSYELPDGQ CCCCCCHHEECCCCC	17.92	28689409
241	Phosphorylation	SSSLEKSYELPDGQV CCCCCHHEECCCCCE	32.33	22108457
250	Phosphorylation	LPDGQVITIGNERFR CCCCCEEEECCCEEC	24.79	46314633
292	Acetylation	KCDIDIRKDLYANNV CCCCEECHHHHHCCC	53.04	54350349
295	Phosphorylation	IDIRKDLYANNVLSG CEECHHHHHCCCCCC	19.88	20563991
307	Phosphorylation	LSGGTTMYPGIADRM CCCCCCCCCCHHHHH	9.01	11887203
316	Acetylation	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	7221457
316	Ubiquitination	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	-
319	Phosphorylation	DRMQKEITALAPSTM HHHHHHHHHHCCCCE	19.40	3972893
324	Phosphorylation	EITALAPSTMKIKII HHHHHCCCCEEEEEE	35.21	9423901
325	Phosphorylation	ITALAPSTMKIKIIA HHHHCCCCEEEEEEC	22.79	27097102
327	Ubiquitination	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	-
327	Acetylation	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	7215597
329	Ubiquitination	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	-
329	Acetylation	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	7692073
339	Phosphorylation	APPERKYSVWIGGSI CCCCCCEEEEECHHH	17.63	30417516
369	Phosphorylation	EYDEAGPSIVHRKCF CCCCCCCCCCCCCCC	36.00	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
34	S	Phosphorylation	Kinase	PRKACA	P00517	GPS
200	S	Phosphorylation	Kinase	PRKACA	P00517	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
45	M	Oxidation	Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.	-
48	M	Oxidation	Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.	-
74	H	Methylation	Methylated at His-74 by SETD3.	-
85	K	Methylation	Monomethylation at Lys-85 (K85me1) regulates actin-myosin interaction and actomyosin-dependent processes.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ACTH_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of ACTH_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.