ACTA_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ACTA_MOUSE
• UniProt AC: P62737
• Protein Name: Actin, aortic smooth muscle
• Gene Name: Acta2
• Organism: Mus musculus (Mouse).
• Sequence Length: 377
• Subcellular Localization: Cytoplasm, cytoskeleton.
• Protein Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells..
• Protein Sequence: MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MCEEEDSTA ------CCCCCCCCE	10.16	6725286
3	Acetylation	-----MCEEEDSTAL -----CCCCCCCCEE	64.22	6725286
35	Phosphorylation	APRAVFPSIVGRPRH CCCCCCHHHCCCCCC	20.59	27180971
46	Oxidation	RPRHQGVMVGMGQKD CCCCCCEEEECCCCC	2.49	23911929
49	Oxidation	HQGVMVGMGQKDSYV CCCEEEECCCCCCCC	3.32	23911929
52	Acetylation	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	36642111
52	Ubiquitination	VMVGMGQKDSYVGDE EEEECCCCCCCCCCH	42.80	27667366
54	Phosphorylation	VGMGQKDSYVGDEAQ EECCCCCCCCCCHHH	28.84	25521595
55	Phosphorylation	GMGQKDSYVGDEAQS ECCCCCCCCCCHHHC	20.70	26824392
62	Phosphorylation	YVGDEAQSKRGILTL CCCCHHHCCCCEEEE	31.22	27087446
63	Ubiquitination	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	27667366
63	Acetylation	VGDEAQSKRGILTLK CCCHHHCCCCEEEEE	42.43	36642155
68	Phosphorylation	QSKRGILTLKYPIEH HCCCCEEEEECEECC	21.20	27600695
70	Ubiquitination	KRGILTLKYPIEHGI CCCEEEEECEECCCC	43.69	-
70	Acetylation	KRGILTLKYPIEHGI CCCEEEEECEECCCC	43.69	36642121
71	Phosphorylation	RGILTLKYPIEHGII CCEEEEECEECCCCC	16.74	26239621
75	Methylation	TLKYPIEHGIITNWD EEECEECCCCCCCHH	33.21	-
79	Phosphorylation	PIEHGIITNWDDMEK EECCCCCCCHHHHHH	28.47	36012721
86	Methylation	TNWDDMEKIWHHSFY CCHHHHHHHHHHHHH	44.45	-
86	Acetylation	TNWDDMEKIWHHSFY CCHHHHHHHHHHHHH	44.45	23954790
105	Phosphorylation	VAPEEHPTLLTEAPL CCCCCCCCCCCCCCC	35.99	28542873
108	Phosphorylation	EEHPTLLTEAPLNPK CCCCCCCCCCCCCCC	32.96	22210690
115	Ubiquitination	TEAPLNPKANREKMT CCCCCCCCCCHHHHH	58.16	27667366
143	Phosphorylation	VAIQAVLSLYASGRT HHHHHHHHHHHCCCC	16.85	19060867
145	Phosphorylation	IQAVLSLYASGRTTG HHHHHHHHHCCCCEE	8.71	36642107
147	Phosphorylation	AVLSLYASGRTTGIV HHHHHHHCCCCEEEE	18.43	-
150	Phosphorylation	SLYASGRTTGIVLDS HHHHCCCCEEEEEEC	32.66	22210690
151	Phosphorylation	LYASGRTTGIVLDSG HHHCCCCEEEEEECC	24.45	22210690
157	Phosphorylation	TTGIVLDSGDGVTHN CEEEEEECCCCCCCC	34.32	28542873
168	Phosphorylation	VTHNVPIYEGYALPH CCCCCCEECCCCHHH	9.38	28464351
171	Phosphorylation	NVPIYEGYALPHAIM CCCEECCCCHHHHHH	7.94	36642093
188	Phosphorylation	DLAGRDLTDYLMKIL HCCCCCHHHHHHHHH	27.21	24899341
190	Phosphorylation	AGRDLTDYLMKILTE CCCCHHHHHHHHHHH	11.94	2001175
193	Ubiquitination	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	-
193	Acetylation	DLTDYLMKILTERGY CHHHHHHHHHHHCCC	31.78	36642167
196	Phosphorylation	DYLMKILTERGYSFV HHHHHHHHHCCCCCE	27.40	46158553
200	Phosphorylation	KILTERGYSFVTTAE HHHHHCCCCCEEHHC	12.73	36642141
201	Phosphorylation	ILTERGYSFVTTAER HHHHCCCCCEEHHCH	18.96	35093905
204	Phosphorylation	ERGYSFVTTAEREIV HCCCCCEEHHCHHHH	20.48	24899341
205	Phosphorylation	RGYSFVTTAEREIVR CCCCCEEHHCHHHHH	22.63	24899341
215	Acetylation	REIVRDIKEKLCYVA HHHHHHHHHHHCEEE	53.75	36642119
217	Ubiquitination	IVRDIKEKLCYVALD HHHHHHHHHCEEEEE	38.56	-
220	Phosphorylation	DIKEKLCYVALDFEN HHHHHHCEEEEECCH	10.13	24351
231	Phosphorylation	DFENEMATAASSSSL ECCHHHHHHCCCCCC	22.80	23737553
234	Phosphorylation	NEMATAASSSSLEKS HHHHHHCCCCCCCHH	28.43	23737553
235	Phosphorylation	EMATAASSSSLEKSY HHHHHCCCCCCCHHE	21.15	23737553
236	Phosphorylation	MATAASSSSLEKSYE HHHHCCCCCCCHHEE	36.13	23737553
237	Phosphorylation	ATAASSSSLEKSYEL HHHCCCCCCCHHEEC	42.36	23737553
241	O-linked_Glycosylation	SSSSLEKSYELPDGQ CCCCCCHHEECCCCC	17.92	36642115
241	Phosphorylation	SSSSLEKSYELPDGQ CCCCCCHHEECCCCC	17.92	25521595
242	Phosphorylation	SSSLEKSYELPDGQV CCCCCHHEECCCCCE	32.33	24925903
251	Phosphorylation	LPDGQVITIGNERFR CCCCCEEEECCCEEC	24.79	46158343
259	Glutathionylation	IGNERFRCPETLFQP ECCCEECCCCHHCCC	3.08	24333276
262	Phosphorylation	ERFRCPETLFQPSFI CEECCCCHHCCCCCC	20.29	22817900
267	Phosphorylation	PETLFQPSFIGMESA CCHHCCCCCCCHHCC	20.79	46158271
281	Phosphorylation	AGIHETTYNSIMKCD CCCCCCCCHHHHCCC	17.84	15569657
293	Ubiquitination	KCDIDIRKDLYANNV CCCCEECHHHHHCCC	53.04	-
293	Acetylation	KCDIDIRKDLYANNV CCCCEECHHHHHCCC	53.04	23236377
305	Phosphorylation	NNVLSGGTTMYPGIA CCCCCCCCCCCCCHH	16.35	21082442
308	Phosphorylation	LSGGTTMYPGIADRM CCCCCCCCCCHHHHH	9.01	-
317	Ubiquitination	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	27667366
317	Acetylation	GIADRMQKEITALAP CHHHHHHHHHHHHCC	41.56	36642179
320	Phosphorylation	DRMQKEITALAPSTM HHHHHHHHHHCCCCE	19.40	25521595
325	Phosphorylation	EITALAPSTMKIKII HHHHHCCCCEEEEEE	35.21	25521595
326	Phosphorylation	ITALAPSTMKIKIIA HHHHCCCCEEEEEEC	22.79	46158475
328	Acetylation	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	36642145
328	Ubiquitination	ALAPSTMKIKIIAPP HHCCCCEEEEEECCC	40.72	27667366
330	Acetylation	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	36642153
330	Ubiquitination	APSTMKIKIIAPPER CCCCEEEEEECCCCC	23.65	27667366
364	Phosphorylation	MWISKQEYDEAGPSI HHHCHHHHCCCCCCC	19.31	28542873
370	Phosphorylation	EYDEAGPSIVHRKCF HHCCCCCCCCCCCCC	36.00	27742792

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ACTA_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
46	M	Oxidation	Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.	23911929
49	M	Oxidation	Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization.	23911929
75	H	Methylation	Methylated at His-75 by SETD3.	-
86	K	Methylation	Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ACTA_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LASP1_MOUSE	Lasp1	physical	15465019

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.