AB4B_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AB4B_ARATH
• UniProt AC: O80725
• Protein Name: ABC transporter B family member 4
• Gene Name: ABCB4
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 1286
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Non-polar distribution in apical cells. Apical (bottom) localization in mature root cells. Basal (top) localization in the root elongation zone.
• Protein Description: Auxin influx transporter that mediates the transport of auxin in roots. Contributes to the basipetal transport in hypocotyls and root tips by establishing an auxin uptake sink in the root cap. Confers sensitivity to 1-N-naphthylphthalamic acid (NPA). Regulates the root elongation, the initiation of lateral roots and the development of root hairs. Can transport IAA, indole-3-propionic acid, NPA syringic acid, vanillic acid and some auxin metabolites, but not 2,4-D and 1-naphthaleneacetic acid..
• Protein Sequence: MASESGLNGDPNILEEVSETKRDKEEEEEVKKTEKKDEEHEKTKTVPFYKLFAFADSFDFLLMILGTLGSIGNGLGFPLMTLLFGDLIDAFGENQTNTTDKVSKVALKFVWLGIGTFAAAFLQLSGWMISGERQAARIRSLYLKTILRQDIAFFDIDTNTGEVVGRMSGDTVLIQDAMGEKVGKAIQLLATFVGGFVIAFVRGWLLTLVMLSSIPLLVMAGALLAIVIAKTASRGQTAYAKAATVVEQTIGSIRTVASFTGEKQAISNYNKHLVTAYKAGVIEGGSTGLGLGTLFLVVFCSYALAVWYGGKLILDKGYTGGQVLNIIIAVLTGSMSLGQTSPCLSAFAAGQAAAYKMFETIERRPNIDSYSTNGKVLDDIKGDIELKDVYFTYPARPDEQIFRGFSLFISSGTTVALVGQSGSGKSTVVSLIERFYDPQAGDVLIDGINLKEFQLKWIRSKIGLVSQEPVLFTASIKDNIAYGKEDATTEEIKAAAELANASKFVDKLPQGLDTMVGEHGTQLSGGQKQRIAVARAILKDPRILLLDEATSALDAESERVVQEALDRIMVNRTTVVVAHRLSTVRNADMIAVIHQGKIVEKGSHTELLKDPEGAYSQLIRLQEEKKSDENAAEEQKMSSIESFKQSSLRKSSLGRSLSKGGSSRGNSSRHSFNMFGFPAGIDGNVVQDQEEDDTTQPKTEPKKVSIFRIAALNKPEIPVLILGSISAAANGVILPIFGILISSVIKAFFQPPKKLKEDTSFWAIIFMVLGFASIIAYPAQTFFFAIAGCKLVQRIRSMCFEKVVHMEVGWFDEPENSSGTIGARLSADAATIRGLVGDSLAQTVQNLSSILAGLIIAFLACWQLAFVVLAMLPLIALNGFLYMKFMKGFSADAKKMYGEASQVANDAVGSIRTVASFCAEDKVMNMYSKKCEGPMKNGIRQGIVSGIGFGFSFFVLFSSYAASFYVGARLVDDGKTTFDSVFRVFFALTMAAMAISQSSSLSPDSSKADVAAASIFAIMDRESKIDPSVESGRVLDNVKGDIELRHVSFKYPARPDVQIFQDLCLSIRAGKTVALVGESGSGKSTVIALLQRFYDPDSGEITLDGVEIKSLRLKWLRQQTGLVSQEPILFNETIRANIAYGKGGDASESEIVSSAELSNAHGFISGLQQGYDTMVGERGIQLSGGQKQRVAIARAIVKDPKVLLLDEATSALDAESERVVQDALDRVMVNRTTIVVAHRLSTIKNADVIAVVKNGVIVEKGKHDTLINIKDGVYASLVQLHLTAAS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MASESGLNGD -----CCCCCCCCCC	44.12	30407730
5	Phosphorylation	---MASESGLNGDPN ---CCCCCCCCCCCC	46.19	19880383
18	Phosphorylation	PNILEEVSETKRDKE CCHHHHHHHHHCCHH	42.71	30407730
20	Phosphorylation	ILEEVSETKRDKEEE HHHHHHHHHCCHHHH	24.71	30407730
94	N-linked_Glycosylation	LIDAFGENQTNTTDK HHHHHCCCCCCCCHH	55.12	-
97	N-linked_Glycosylation	AFGENQTNTTDKVSK HHCCCCCCCCHHHHH	31.08	-
427	Phosphorylation	QSGSGKSTVVSLIER CCCCCHHHHHHHHHH	28.54	23328941
500	N-linked_Glycosylation	KAAAELANASKFVDK HHHHHHHHHHHHHHH	58.32	-
502	Phosphorylation	AAELANASKFVDKLP HHHHHHHHHHHHHCC	26.83	23328941
571	N-linked_Glycosylation	ALDRIMVNRTTVVVA HHHHHCCCCCEEEEE	20.43	-
638	Phosphorylation	AAEEQKMSSIESFKQ HHHHHHHHHHHHHHH	34.89	23111157
639	Phosphorylation	AEEQKMSSIESFKQS HHHHHHHHHHHHHHH	27.20	20374526
642	Phosphorylation	QKMSSIESFKQSSLR HHHHHHHHHHHHHCC	35.75	19880383
646	Phosphorylation	SIESFKQSSLRKSSL HHHHHHHHHCCHHHH	31.75	15308754
647	Phosphorylation	IESFKQSSLRKSSLG HHHHHHHHCCHHHHC	29.69	15308754
656	Phosphorylation	RKSSLGRSLSKGGSS CHHHHCCHHCCCCCC	34.89	25561503
658	Phosphorylation	SSLGRSLSKGGSSRG HHHCCHHCCCCCCCC	30.42	25561503
662	Phosphorylation	RSLSKGGSSRGNSSR CHHCCCCCCCCCCCC	26.07	25561503
666	N-linked_Glycosylation	KGGSSRGNSSRHSFN CCCCCCCCCCCCCCC	35.38	-
671	Phosphorylation	RGNSSRHSFNMFGFP CCCCCCCCCCCCCCC	19.73	15308754
816	N-linked_Glycosylation	GWFDEPENSSGTIGA ECCCCCCCCCCCCCC	52.79	-
846	N-linked_Glycosylation	SLAQTVQNLSSILAG HHHHHHHHHHHHHHH	37.24	-
1094	Phosphorylation	IALLQRFYDPDSGEI HHHHHHHCCCCCCEE	28.84	19880383
1131	N-linked_Glycosylation	SQEPILFNETIRANI CCCCCCCCHHHHHHH	41.90	-
1230	N-linked_Glycosylation	ALDRVMVNRTTIVVA HHHHHHCCCCEEEEE	19.93	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of AB4B_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AB4B_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AB4B_ARATH !!

Protein-Protein Interaction

Oops, there are no PPI records of AB4B_ARATH !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Quantitative phosphoproteomic analysis of plasma membrane proteinsreveals regulatory mechanisms of plant innate immune responses.";
Nuehse T.S., Bottrill A.R., Jones A.M.E., Peck S.C.;
Plant J. 51:931-940(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-642, AND MASSSPECTROMETRY.
PubMed: 17651370

"Phosphoproteomics of the Arabidopsis plasma membrane and a newphosphorylation site database.";
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
Plant Cell 16:2394-2405(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-642 AND SER-671, ANDMASS SPECTROMETRY.
PubMed: 15308754

"Large-scale analysis of in vivo phosphorylated membrane proteins byimmobilized metal ion affinity chromatography and mass spectrometry.";
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
Mol. Cell. Proteomics 2:1234-1243(2003).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-642; SER-646 ANDSER-647, AND MASS SPECTROMETRY.
PubMed: 14506206