4F2_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: 4F2_RAT
• UniProt AC: Q794F9
• Protein Name: 4F2 cell-surface antigen heavy chain
• Gene Name: Slc3a2 {ECO:0000312|RGD:3073}
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 527
• Subcellular Localization: Apical cell membrane ; Single-pass type II membrane protein . Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (By similarity). Localized to the plasma membrane when associated with SLC7A5 or SLC7A8 (By sim
• Protein Description: Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. When associated with LAPTM4B, recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (By similarity)..
• Protein Sequence: MSQDTEVDMKDVELNELEPEKQPMNAADGAAAGEKNGLVKIKVAEDEAEAGVKFTGLSKEELLKVAGSPGWVRTRWALLLLFWLGWLGMLAGAVVIIVRAPRCRELPVQRWWHKGALYRIGDLQAFVGPEARGIAGLKNHLEYLSTLKVKGLVLGPIHKNQKDEVNETDLKQIDPDLGSQEDFKDLLQSAKKKSIHIILDLTPNYKGQNAWFLPPQADIVATKMKEALSSWLQDGVDGFQVRDVGKLANASLYLAEWQNITKNFSEDRLLIAGTASSDLQQIVNILESTSDLLLTSSYLSQPVFTGEHAELLVIKYLNATGSRWCSWSVSQAGLLTSFIPAQFLRLYQLLLFTLPGTPVFSYGDELGLQAVALPGQPMEAPFMLWNESSNSQTSSPVSLNMTVKGQNEDPGSLLTQFRRLSDLRGKERSLLHGDFDALSSSSGLFSYVRHWDQNERYLVVLNFQDVGLSARVGASNLPAGISLPASANLLLSTDSTRLSREEGTSLSLENLSLNPYEGLLLQFPFVA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSQDTEVDM ------CCCCCCCCH	36.44	27097102
5	Phosphorylation	---MSQDTEVDMKDV ---CCCCCCCCHHHC	30.21	27097102
10	Acetylation	QDTEVDMKDVELNEL CCCCCCHHHCCHHHC	54.91	83034461
21	Ubiquitination	LNELEPEKQPMNAAD HHHCCCCCCCCCHHC	71.11	-
21	Acetylation	LNELEPEKQPMNAAD HHHCCCCCCCCCHHC	71.11	55699617
35	Ubiquitination	DGAAAGEKNGLVKIK CCCCCCCCCCEEEEE	56.23	-
42	Ubiquitination	KNGLVKIKVAEDEAE CCCEEEEEEECCHHH	29.67	-
53	Ubiquitination	DEAEAGVKFTGLSKE CHHHHCCEECCCCHH	35.97	-
55	Phosphorylation	AEAGVKFTGLSKEEL HHHCCEECCCCHHHH	31.34	28432305
58	Phosphorylation	GVKFTGLSKEELLKV CCEECCCCHHHHHHH	39.09	30240740
59	Acetylation	VKFTGLSKEELLKVA CEECCCCHHHHHHHH	61.32	22902405
59	Ubiquitination	VKFTGLSKEELLKVA CEECCCCHHHHHHHH	61.32	-
148	Acetylation	LEYLSTLKVKGLVLG HHHHHHCCCCEEEEC	41.82	22902405
162	Acetylation	GPIHKNQKDEVNETD CCCCCCCCCCCCHHH	66.31	22902405
166	N-linked_Glycosylation	KNQKDEVNETDLKQI CCCCCCCCHHHHHHC	44.39	-
179	Phosphorylation	QIDPDLGSQEDFKDL HCCCCCCCHHHHHHH	37.38	28689409
184	Acetylation	LGSQEDFKDLLQSAK CCCHHHHHHHHHHHH	60.84	22902405
191	Acetylation	KDLLQSAKKKSIHII HHHHHHHHHCCEEEE	67.01	22902405
249	N-linked_Glycosylation	RDVGKLANASLYLAE ECHHHHHCHHHHHHH	40.26	-
259	N-linked_Glycosylation	LYLAEWQNITKNFSE HHHHHHHHHCCCCCC	44.99	-
263	N-linked_Glycosylation	EWQNITKNFSEDRLL HHHHHCCCCCCCCEE	36.71	-
300	Phosphorylation	LLTSSYLSQPVFTGE HHCHHHHCCCCCCCC	24.97	-
318	N-linked_Glycosylation	LLVIKYLNATGSRWC EEEEEECCCCCCCCC	32.16	-
386	N-linked_Glycosylation	EAPFMLWNESSNSQT CCCEEEEECCCCCCC	35.00	-
400	N-linked_Glycosylation	TSSPVSLNMTVKGQN CCCCEEEEEEEECCC	19.30	-
421	Phosphorylation	LTQFRRLSDLRGKER HHHHHHHHHCCCCCH	32.56	22673903
510	N-linked_Glycosylation	GTSLSLENLSLNPYE CCCCEEECCCCCCCC	39.98	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of 4F2_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
300	S	Phosphorylation	Phosphorylation on Ser-300 and on Ser-421 by ecto-protein kinases favors heterotypic cell-cell interactions.	-
421	S	Phosphorylation	Phosphorylation on Ser-300 and on Ser-421 by ecto-protein kinases favors heterotypic cell-cell interactions.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of 4F2_RAT !!

Protein-Protein Interaction

Oops, there are no PPI records of 4F2_RAT !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.