14334_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: 14334_ARATH
• UniProt AC: P46077
• Protein Name: 14-3-3-like protein GF14 phi
• Gene Name: GRF4
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 267
• Subcellular Localization: Nucleus . Cytoplasm . Translocates from the cytosol to the nucleus when phosphorylated.
• Protein Description: Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes..
• Protein Sequence: MAAPPASSSAREEFVYLAKLAEQAERYEEMVEFMEKVAEAVDKDELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNDDHVTTIRDYRSKIESELSKICDGILKLLDTRLVPASANGDSKVFYLKMKGDYHRYLAEFKTGQERKDAAEHTLTAYKAAQDIANAELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQDESPEEIKEAAAPKPAEEQKEI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAPPASSS ------CCCCCCCCC	28.77	22223895
55	Phosphorylation	VEERNLLSVAYKNVI HHHHHHHHHHHHHHH	14.10	25561503
73	Phosphorylation	RASWRIISSIEQKEE HHHHHHHHHHHHHHH	23.92	-
89	Phosphorylation	RGNDDHVTTIRDYRS CCCCCCCHHHHHHHH	17.06	19880383
94	Phosphorylation	HVTTIRDYRSKIESE CCHHHHHHHHHHHHH	13.62	19880383
196	Phosphorylation	FYYEILNSPDRACNL HHHHHHCCHHHHHHH	25.40	-
217	Phosphorylation	EAIAELDTLGEESYK HHHHHHHCCCCHHHC	49.65	25368622
222	Phosphorylation	LDTLGEESYKDSTLI HHCCCCHHHCCHHHH	32.93	25368622
238	Phosphorylation	QLLRDNLTLWTSDMQ HHHHCCCCCCCCCCC	26.89	23776212
241	Phosphorylation	RDNLTLWTSDMQDES HCCCCCCCCCCCCCC	20.26	23776212
242	Phosphorylation	DNLTLWTSDMQDESP CCCCCCCCCCCCCCH	21.36	23776212
248	Phosphorylation	TSDMQDESPEEIKEA CCCCCCCCHHHHHHH	45.99	30291188

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of 14334_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of 14334_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of 14334_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AI5L6_ARATH	ABF3	physical	21085673
CDPK1_ARATH	CPK1	physical	22737156
PTR2_ARATH	PTR2	physical	22737156
CINV1_ARATH	CINV1	physical	25256212

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale Arabidopsis phosphoproteome profiling reveals novelchloroplast kinase substrates and phosphorylation networks.";
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,Grossmann J., Gruissem W., Baginsky S.;
Plant Physiol. 150:889-903(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, AND MASSSPECTROMETRY.
PubMed: 19376835

"Phosphoproteomic analysis of nuclei-enriched fractions fromArabidopsis thaliana.";
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A.,Andreasson E., Rathjen J.P., Peck S.C.;
J. Proteomics 72:439-451(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, AND MASSSPECTROMETRY.
PubMed: 19245862