Protein Name:
DNA topoisomerase 3-beta-1
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UniprotKB/SwissProt ID: Q9Z321 (Q9Z321)
Gene Name:
Top3b
Organism: Mus musculus (Mouse)
Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity
Other Modifications: View all modification sites in dbPTM
Protein Subcellular Localization:
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Graphical Visualization of S-nitrosylation Sites:
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