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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: DNA-directed RNA polymerase I subunit 1

UniprotKB/SwissProt ID: Q9SVY0 (Q9SVY0)

Gene Name: NRPA1

Organism: Arabidopsis thaliana (Mouse-ear cress)

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from NRPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Nucleus

Graphical Visualization of S-nitrosylation Sites:
InterPro ID Domain Name
IPR047107 DNA-dir_RNA_pol1_lsu_C
IPR015699 DNA-dir_RNA_pol1_lsu_N
IPR045867 DNA-dir_RpoC_beta_prime
IPR000722 RNA_pol_asu
IPR006592 RNA_pol_N
IPR007080 RNA_pol_Rpb1_1
IPR007066 RNA_pol_Rpb1_3
IPR042102 RNA_pol_Rpb1_3_sf
IPR007083 RNA_pol_Rpb1_4
IPR007081 RNA_pol_Rpb1_5
IPR044893 RNA_pol_Rpb1_clamp_domain
IPR038120 Rpb1_funnel_sf

The S-nitrosylation sites of Q9SVY0

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site PubMed ID
1 79 KLGPKDDKQA C NSCGQLKLAC