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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

UniprotKB/SwissProt ID: Q91VD9 (Q91VD9)

Gene Name: Ndufs1

Organism: Mus musculus (Mouse)

Function: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:27799543, PubMed:32072193, PubMed:38575788). Essential for catalysing the entry and efficient transfer of electrons within complex I (PubMed:27799543). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (PubMed:27799543)

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Mitochondrion inner membrane

Graphical Visualization of S-nitrosylation Sites:
InterPro ID Domain Name
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR054351 NADH_UbQ_OxRdtase_ferredoxin
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
IPR015405 NDUFS1-like_C
IPR050123 Prok_molybdopt-oxidoreductase

The S-nitrosylation sites of Q91VD9

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site PubMed ID
1 367 LLNKVDSDNL C TEEIFPTEGA  CCCCCCCCHH H CCCCCCCCCE  5.88% 21278135
2 463 QDIASGRHSF C EVLKDAKKPM  CCCCCCCCHH H CCCCCCCCCE  3.86% 21278135
3 554 MLFLLGADGG C ITRQDLPKDC  CCCCCCCCHH H CCCCCCCCCE  3.34% 21278135
4 564 CITRQDLPKD C FIVYQGHHGD  CCCCCCCCHH H CCCCCCCCCE  2.9% 21278135
5 64 EKVGMQIPRF C YHERLSVAGN  CCCCCCCCHH H CCCCCCCCCE  3.41% 21278135
6 710 ISRASQTMAK C VKAVTEGAQA  CCCCCCCCHH H CCCCCCCCCE  2.49% 21278135
7 727 GAQAVEEPSI C ----------  CCCCCCCCHH H CCCCCCCCCE  5.53% 21278135
8 75 YHERLSVAGN C RMCLVEIEKA  CCCCCCCCHH H CCCCCCCCCE  2.61% 21278135
9 78 RLSVAGNCRM C LVEIEKAPKV  CCCCCCCCHH H CCCCCCCCCE  1.67%
10 92 IEKAPKVVAA C AMPVMKGWNI  CCCCCCCCHH H CCCCCCCCCE  2.1% 21278135