Protein Name:
7SK snRNA methylphosphate capping enzyme
|
UniprotKB/SwissProt ID: Q7L2J0 (Q7L2J0)
Gene Name:
MEPCE
Organism: Homo sapiens (Human)
Function: S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it (PubMed:17643375, PubMed:19906723, PubMed:30559425). Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PubMed:17643375). The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (PubMed:28254838). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex (PubMed:19906723, PubMed:38100593). MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center (PubMed:19906723). Also required for stability of U6 snRNAs (PubMed:38100593)
Other Modifications: View all modification sites in dbPTM
Protein Subcellular Localization: Nucleus
|
|
|
Graphical Visualization of S-nitrosylation Sites:
|
|
The S-nitrosylation sites of Q7L2J0
|
| No. |
Position |
S-nitrosylated Peptide |
Secondary Structure of S-nitrosylated Peptide |
Solvent Accessibility of nitrosylated Site |
PubMed ID |
| 1 |
153 |
GGGGGKRRNS C NVGGGGGGFK |
|
|
|
| 2 |
244 |
DITDPLSLNT C TDEGHVVLAS |
|
|
|
| 3 |
324 |
QPYELNTAIN C RDEVVSPLPS |
|
|
|
| 4 |
419 |
QQRKFQYGNY C KYYGYRNPSC |
|
|
|
| 5 |
522 |
EGTTTVRKRS C FPASLTASRG |
|
|
|
| 6 |
54 |
RGGTERGPGR C APSAGSPAAA |
|
|
|
|
