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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Large ribosomal subunit protein uL2

UniprotKB/SwissProt ID: P60422 (P60422)

Gene Name: rplB

Organism: Escherichia coli (strain K12)

Function: One of the primary rRNA binding proteins (PubMed:3298242). Located near the base of the L1 stalk, it is probably also mobile. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation (PubMed:8722025). It has been suggested to have peptidyltransferase activity; this is highly controversial In the E.coli 70S ribosome in the initiation state it has been modeled to make several contacts with the 16S rRNA (forming bridge B7b, PubMed:12809609); these contacts are broken in the model with bound EF-G

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization:

Graphical Visualization of S-nitrosylation Sites:
InterPro ID Domain Name
IPR012340 NA-bd_OB-fold
IPR014722 Rib_uL2_dom2
IPR002171 Ribosomal_uL2
IPR005880 Ribosomal_uL2_bac/org-type
IPR022669 Ribosomal_uL2_C
IPR022671 Ribosomal_uL2_CS
IPR014726 Ribosomal_uL2_dom3
IPR022666 Ribosomal_uL2_RNA-bd_dom
IPR008991 Translation_prot_SH3-like_sf

The S-nitrosylation sites of P60422

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site PubMed ID
1 188 SGEMRKVEAD C RATLGEVGNA  CCEEECCCCC C CCCEEEEEEC  4.03% 22178444
19483679
2 6 -----MAVVK C KPTSPGRRHV  CCEEECCCCC C CCCEEEEEEC  4.28% 22178444
19483679