UniprotKB/SwissProt ID: P14925 (P14925)
Gene Name:
Pam
Organism: Rattus norvegicus (Rat)
Function: Bifunctional enzyme that catalyzes amidation of the C-terminus of proteins (PubMed:10079066, PubMed:36880254, PubMed:7893699). Alpha-amidation is present at the C-terminus of many endocrine hormones and neuropeptides and is required for their activity (By similarity). C-terminal amidation also takes place in response to protein fragmentation triggered by oxidative stress, promoting degradation of amidated protein fragments by the proteasome (By similarity). Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme (PubMed:10079066). The first step, catalyzed by peptidyl alpha-hydroxylating monooxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (PubMed:10079066). The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (PubMed:10079066). Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (PubMed:10079066)
Other Modifications: View all modification sites in dbPTM
Protein Subcellular Localization: Cytoplasmic vesicle, secretory vesicle membrane. Membrane. Membrane. Secreted. Secreted. Membrane. Secreted. Secreted
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