Protein Name:
Bifunctional glutathionylspermidine synthetase/amidase
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UniprotKB/SwissProt ID: P0AES0 (P0AES0)
Gene Name:
gss
Organism: Escherichia coli (strain K12)
Function: Catalyzes the formation of an amide bond between glutathione (GSH) and spermidine coupled with hydrolysis of ATP; also catalyzes the opposing reaction, i.e. the hydrolysis of glutathionylspermidine (Gsp) back to glutathione and spermidine. The amidase active site can also hydrolyze Gsp-disulfide (Gsp-S-S-Gsp) to Gsp-SG and Gsp S-thiolated proteins (GspSSPs) to GSH S-thiolated protein (GSSPs). Likely acts synergistically with glutaredoxin to regulate the redox environment of E.coli and defend against oxidative damage. In vitro, the amidase active site also catalyzes hydrolysis of amide and ester derivatives of glutathione (e.g. glutathione ethyl ester and glutathione amide) but lacks activity toward acetylspermidine (N1 and N8) and acetylspermine (N1)
Other Modifications: View all modification sites in dbPTM
Protein Subcellular Localization:
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Graphical Visualization of S-nitrosylation Sites:
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