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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Phosphoenolpyruvate-protein phosphotransferase

UniprotKB/SwissProt ID: P08839 (P08839)

Gene Name: ptsI

Organism: Escherichia coli (strain K12)

Function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr) (PubMed:12705838, PubMed:17053069, PubMed:7876255). Can also use (Z)-3-fluoro-PEP (ZFPEP), (Z)-3-methyl-PEP (ZMePEP), (Z)-3-chloro-PEP (ZClPEP) and (E)-3-chloro-PEP (EClPEP) as alternative phosphoryl donors (PubMed:12705838)

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm

Graphical Visualization of S-nitrosylation Sites:
InterPro ID Domain Name
IPR008279 PEP-util_enz_mobile_dom
IPR050499 PEP-utilizing_PTS_enzyme
IPR018274 PEP_util_AS
IPR000121 PEP_util_C
IPR023151 PEP_util_CS
IPR036637 Phosphohistidine_dom_sf
IPR024692 PTS_EI
IPR006318 PTS_EI-like
IPR008731 PTS_EIN
IPR036618 PtsI_HPr-bd_sf
IPR015813 Pyrv/PenolPyrv_kinase-like_dom
IPR040442 Pyrv_kinase-like_dom_sf

The S-nitrosylation sites of P08839

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site PubMed ID
1 324 FAAYKAVAEA C GSQAVIVRTM  CCCCCCCCCC C HHCHHHHCCC  3.97% 22178444
19483679