Protein Name:
DNA topoisomerase 3-beta-1
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UniprotKB/SwissProt ID: O95985 (O95985)
Gene Name:
TOP3B
Organism: Homo sapiens (Human)
Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity
Other Modifications: View all modification sites in dbPTM
Protein Subcellular Localization:
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Graphical Visualization of S-nitrosylation Sites:
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The S-nitrosylation sites of O95985
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| No. |
Position |
S-nitrosylated Peptide |
Secondary Structure of S-nitrosylated Peptide |
Solvent Accessibility of nitrosylated Site |
PubMed ID |
| 1 |
190 |
ARQELDLRIG C AFTRFQTKYF |
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| 2 |
217 |
LDSSLISFGP C QTPTLGFCVE |
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| 3 |
470 |
SVPLEESLPT C QRGDAFPVGE |
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