Protein Name:
Eukaryotic translation initiation factor 5B
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UniprotKB/SwissProt ID: O60841 (O60841)
Gene Name:
EIF5B
Organism: Homo sapiens (Human)
Function: Plays a role in translation initiation (PubMed:10659855, PubMed:35732735). Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon (PubMed:10659855, PubMed:35732735). Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex (PubMed:12569173, PubMed:35732735). Is released after formation of the 80S initiation complex (PubMed:35732735). Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes (PubMed:10659855, PubMed:35732735). In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit (PubMed:10659855)
Other Modifications: View all modification sites in dbPTM
Protein Subcellular Localization: Cytoplasm
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Graphical Visualization of S-nitrosylation Sites:
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The S-nitrosylation sites of O60841
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| No. |
Position |
S-nitrosylated Peptide |
Secondary Structure of S-nitrosylated Peptide |
Solvent Accessibility of nitrosylated Site |
PubMed ID |
| 1 |
1092 |
EEFKHIAVFP C KIKILPQYIF |
CCCCCCCCCC C CCCCCCCHHH |
4.9% |
2212679
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| 2 |
1126 |
AGQVKQGTPM C VPSKNFVDIG |
CCCCCCCCCC C CCCCCCCHHH |
4.24% |
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| 3 |
1158 |
VDVAKKGQEV C VKIEPIPGES |
CCCCCCCCCC C CCCCCCCHHH |
2.25% |
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| 4 |
470 |
SKEVSESMEL C AAVEVMEQGV |
CCCCCCCCCC C CCCCCCCHHH |
1.13% |
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| 5 |
749 |
ESINLLKSKK C PFIVALNKID |
CCCCCCCCCC C CCCCCCCHHH |
3.7% |
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| 6 |
853 |
QTMLSKRLAH C EELRAQVMEV |
CCCCCCCCCC C CCCCCCCHHH |
3.46% |
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