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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: E3 ubiquitin-protein ligase Midline-1

UniprotKB/SwissProt ID: O15344 (O15344)

Gene Name: MID1

Organism: Homo sapiens (Human)

Function: Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle

Graphical Visualization of S-nitrosylation Sites:
InterPro ID Domain Name
IPR001870 B30.2/SPRY
IPR043136 B30.2/SPRY_sf
IPR003649 Bbox_C
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR017903 COS_domain
IPR050617 E3_ligase_FN3/SPRY
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR047095 MID1_Bbox1_Zfn
IPR027727 MID1_Bbox2_Zfn
IPR040859 Midline-1_COS
IPR003877 SPRY_dom
IPR027370 Znf-RING_euk
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS

The S-nitrosylation sites of O15344

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site PubMed ID
1 266 SRQEAKLTEE C DLLIEIIQQR   
2 580 KNSASWALCR C NNNWVVRHNS   
3 647 VCPTFTVWNK C LTIITGLPIP