| PTM Description | Alanine amide |
| Modified Amino Acid | A |
| Position of Modification on Amino Acid | protein backbone |
| Position in the Polypeptide | C-terminal carboxyl group |
| Correction Formula | H1;N1;O-1 |
| Monoisotopic Mass Difference | 0.984016 |
| Average Mass Difference | 0.98 |
| Localization | extracellular and lumenal localisation [Detail] |
| RESID ID | AA0081 |
| Formula Structure |  |
| Substrate Site Specificity |
| Number of Experimentally Validated Sites | 57 |
| The Matrix of Positional Amino Acid Frequency Surrounding Modified Site | | Pos. | -6 | -5 | -4 | -3 | -2 | -1 | 0 | | A | 0.06 | 0.06 | 0.03 | 0.03 | 0.03 | 0.03 | 0 | | R | 0.03 | 0.06 | 0 | 0.06 | 0 | 0.12 | 0 | | N | 0.15 | 0.06 | 0.09 | 0 | 0.09 | 0.06 | 0 | | D | 0 | 0.18 | 0 | 0 | 0.03 | 0.06 | 0 | | C | 0.06 | 0 | 0 | 0 | 0.15 | 0.15 | 0 | | G | 0 | 0.03 | 0.06 | 0.09 | 0.21 | 0 | 0 | | E | 0.06 | 0.09 | 0 | 0 | 0.03 | 0.06 | 0 | | Q | 0.06 | 0 | 0.03 | 0 | 0.06 | 0.03 | 0 | | H | 0.06 | 0 | 0 | 0.18 | 0 | 0 | 0 | | I | 0.03 | 0.06 | 0.06 | 0.06 | 0 | 0.09 | 0 | | L | 0 | 0 | 0.24 | 0.12 | 0.12 | 0.3 | 0 | | K | 0.18 | 0.18 | 0.27 | 0.27 | 0.03 | 0.09 | 0 | | M | 0 | 0 | 0.03 | 0.12 | 0 | 0 | 0 | | F | 0 | 0 | 0 | 0 | 0 | 0 | 0 | | P | 0.15 | 0.09 | 0.03 | 0 | 0.06 | 0 | 0 | | S | 0.06 | 0.03 | 0.03 | 0.03 | 0.06 | 0 | 0 | | T | 0 | 0 | 0.03 | 0 | 0.03 | 0 | 0 | | W | 0.06 | 0 | 0 | 0 | 0 | 0 | 0 | | Y | 0.03 | 0.09 | 0 | 0.03 | 0 | 0 | 0 | | V | 0 | 0.06 | 0.09 | 0 | 0.09 | 0 | 0 |
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| Sequence Logo | 
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| Average Solvent Accessibility Surrounding Modified Site |  |
| Secondary Structure Logo |  |