| PTM Description | N-acetylmethionine |
| Modified Amino Acid | M |
| Position of Modification on Amino Acid | protein backbone |
| Position in the Polypeptide | N-terminal amino group |
| Correction Formula | C2;H2;O1 |
| Monoisotopic Mass Difference | 42.0106 |
| Average Mass Difference | 42.04 |
| Localization | cytoplasm, nucleus, mitochondrial matrix, etc. [Detail] |
| RESID ID | AA0049 |
| Formula Structure |  |
| Substrate Site Specificity |
| Number of Experimentally Validated Sites | 1150 |
| The Matrix of Positional Amino Acid Frequency Surrounding Modified Site | | Pos. | 0 | +1 | +2 | +3 | +4 | +5 | +6 | | A | 0 | 0.02 | 0.11 | 0.1 | 0.09 | 0.09 | 0.1 | | R | 0 | 0 | 0.02 | 0.01 | 0.01 | 0.01 | 0.04 | | N | 0 | 0.1 | 0.05 | 0.06 | 0.03 | 0.04 | 0.04 | | D | 0 | 0.27 | 0.06 | 0.06 | 0.05 | 0.06 | 0.05 | | C | 0 | 0 | 0.01 | 0.01 | 0.02 | 0.01 | 0.03 | | G | 0 | 0.01 | 0.09 | 0.07 | 0.1 | 0.08 | 0.07 | | E | 0 | 0.36 | 0.08 | 0.08 | 0.09 | 0.09 | 0.1 | | Q | 0 | 0.03 | 0.05 | 0.05 | 0.05 | 0.06 | 0.04 | | H | 0 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.02 | | I | 0 | 0.01 | 0.03 | 0.05 | 0.05 | 0.04 | 0.04 | | L | 0 | 0.04 | 0.06 | 0.1 | 0.07 | 0.09 | 0.09 | | K | 0 | 0.01 | 0.06 | 0.04 | 0.06 | 0.06 | 0.06 | | M | 0 | 0.03 | 0.01 | 0.01 | 0.02 | 0.01 | 0.01 | | F | 0 | 0.04 | 0.02 | 0.04 | 0.03 | 0.02 | 0.02 | | P | 0 | 0 | 0.11 | 0.07 | 0.07 | 0.08 | 0.07 | | S | 0 | 0.01 | 0.11 | 0.11 | 0.09 | 0.12 | 0.07 | | T | 0 | 0.03 | 0.06 | 0.06 | 0.06 | 0.05 | 0.06 | | W | 0 | 0 | 0 | 0.01 | 0 | 0 | 0 | | Y | 0 | 0.01 | 0.01 | 0.02 | 0.02 | 0.01 | 0.02 | | V | 0 | 0.02 | 0.05 | 0.05 | 0.06 | 0.06 | 0.06 |
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| Sequence Logo | 
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| Average Solvent Accessibility Surrounding Modified Site |  |
| Secondary Structure Logo |  |